赫赛汀联合丝裂霉素C对人膀胱癌T24细胞生长的抑制作用

目的 探讨赫赛汀(Herceptin,HER)联合丝裂霉素C(MMC)对HER-2/neu基因高表达人膀胱癌细胞株生长的抑制作用.方法 应用免疫细胞化学法、逆转录-聚合酶链反应(RT-PCR)法检测膀胱癌T24细胞株HER-2/neu的表达;采用噻唑蓝(MTT)比色法测定不同浓度HER(10、20、40、80、160μg/L)、MMC(4、8、16、32、64μg/L)及联合用药对人膀胱癌T24细胞株体外生长的抑制率并进行比较.结果 应用免疫细胞化学方法及RT-PCR法检测证实HER-2/neu在膀胱尿路上皮癌T24细胞株高表达;单用HER于72 h出现细胞抑制(72 h时各浓度组分别与48 h时比较,均P<0.05);HER和MMC联用在24、48、72 h基本上均表现为协同作用(q值1.264～3.473),在96 h呈相加作用(q值0.913～1.138).结论 HER-2/neu在膀胱尿路上皮癌T24细胞株高表达;HER单药对T24细胞有轻度抑制作用,且起效慢(72 h);与MMC联合应用能协同抑制T24细胞生长.

Inhibitory effects of Herceptin combined with mitomycin C on human bladder urothelial carcinoma T24 lines

Objective To explore the anti-tumor effects of Herceptin and mitomycin C (MMC) on human bladder urothelial carcinoma T24 lines. Methods The SP immunohistochemical method and RT-PCR method were used to detect the expression of HER-2/neu in human bladder urothelial carcinoma T24 lines. MTT method was applied to assay the growth inhibition rate of various concentrations of Hercep-tin (10,20,40,80,and 160 μg/L),MMC (4,8,16,32,and 64 μg/L),Herceptin combined with MMC on human bladder urothelial carcinoma T24 lines. Results There was high expression of HER-2/neu in hu-man bladder urothelial carcinoma T24 lines by immunocytochemistry and RT-PCR. Herceptin slightly in-hibited proliferation of human bladder urothelial carcinoma T24 lines at the 72nd h as compared with that at the 48th h, all P<0.05). The combined use of herceptine and MCC exerted the synergistic effects at the 24th,48th,and 72nd h (q value: 1. 264 to 3. 473),and an additive effect was obtained at the 96th h (q value:0.913 to 1. 138). Conclusion Her-2/neu was overexpressed in human bladder urothelial carcino-ma T24 lines. Herceptin slightly inhibited proliferation of T24 lines and exerted the effects slowly. There was a synergistic effect to inhibit proliferation of T24 lines by combined use of herceptine and MMC.