PCNA与Caspase-3在胆囊癌组织中的表达及其临床意义

目的 探讨增殖细胞核抗原(PCNA)和半胱氨酸天冬氨酸蛋白酶3(Caspase-3)在胆囊癌组织中的表达及临床意义.方法 采用免疫组化检测42例胆囊癌组织和10例慢性胆囊炎组织石蜡切片中PCNA和Caspase-3的表达水平,并分析与临床病理特征之间的关系.结果 (1)PCNA的阳性表达率在胆囊癌及慢性胆囊炎组分别为64.29%和30‰两组间差异有显著性(P<0.05);Caspase-3在胆囊癌及慢性胆囊炎组分别为30.95%和70%,两组间差异有显著性(P<0.05).(2)胆囊癌组织中,PCNA和Caspase-3表达水平在性别、年龄方面差异无统计学意义(P>0.05),但与淋巴结转移、癌组织分化程度及病理TNM分期有关(P<0.05).(3)PCNA和Caspase-3的表达呈负相关(rs=-0.62,P<0.01).结论 PCNA和Caspase-3的表达在胆囊癌组织中呈负相关,且两者表达水平与胆囊癌淋巴结转移、癌组织分化程度及病TNM分期有关,提示PCNA和Caspase-3是反映胆囊癌生物学行为的重要指标.

Expression and clinical significance of PCNA and Caspase-3 in gallbladder carcinoma tissues

Objective To explore the expressions of PCNA and Caspase-3 in gallbladder carcinoma tissues and their clinical significance.Methods Immunohistochemical method was used to detect the expressions of PCNA and Caspase-3 in paraffin-embedded tissues in 42 cases of gallbladder carcinoma and 10 cases of chronic cholecystitis.A statistical analysis of the expressions of PCNA and Caspase-3 was made,and the relationship between their expressions and the clinical and pathological characteristics were analyzed.Results(1) The positive expression rates of PCNA in gallbladder carcinoma and chronic cholecystitis groups were 64.29% and 30% respectively,which were significantly different between the two groups(P <0.05);The expression rates of Caspase-3 in gallbladder carcinoma and chronic cholecystitis groups were respectively 30.95% and 70%,which were significantly different(P <0.05).(2) In gallbladder carcinoma tissues,the differences on the expressions of gender and age were not statistically significant(P >0.05),but the expressions of PCNA and Caspase-3 were associated with lymph node metastasis,carcinoma tissue differentiation and pathology of TNM stage(P <0.05).(3) The expressions of PCNA and Caspase-3 were negatively correlated(rs=-0.62,P <0.01).Conclusion The expressions of PCNA and Caspase-3 in gallbladder carcinoma tissues are negatively correlated,and both expressions are connected with lymph node metastasis,carcinoma tissue differentiation and pathologic TNM stage,suggesting that PCNA and Caspase-3 are important indicators which reflected biological behaviors of gallbladder carcinoma.