| 茶多酚诱导肝癌细胞凋亡 |
| |
| 引用本文： | 吴勤,付体权,冯传前.茶多酚诱导肝癌细胞凋亡[J].解放军医学杂志,2001,26(2):110-112,F004. |
| |
| 作者姓名： | 吴勤 付体权 冯传前 |
| |
| 作者单位： | 北京解放军第302医院 |
| |
| 基金项目： | 军队医药卫生科研基金资助课题!(编号 1996M14 8) |
| |
| 摘 要： | 为进一步研究茶多酚诱导体外培养的肝癌细胞株HepG-II细胞发生凋亡的作用，采用MTT法、形态学观察、琼脂糖凝胶电泳和末端脱氧核苷酸转移标记法观察被茶多酚处理后的ＨepG－II细胞的形态学和生化等指标的变化。MTT法研究结果显示，当茶多酚浓度为250μg/ml时即时诱导HepG-II细胞凋亡，并与浓度呈正相关；当茶多酚浓度＞2000μg/ml时，抑制率增强的幅度明显减慢。透射电镜下观察到核染色质浓集呈块并可见凋亡小体；荧光染色在荧光显微镜下可见部分细胞核或细胞质内出现致密浓染的黄绿色块状和颗粒状荧光等凋亡细胞的形态学改变。琼脂糖凝胶电泳呈典型的DNA梯形图像。末端脱氧核苷酸转移标记法进一步证实茶多酚可诱导HepG-II细胞的凋亡。提示茶多酚可诱导体外培养的肝癌细胞株HepG-II细胞发生凋亡，具有抗肝癌的作用。
|
| 关 键 词： | 茶多酚 肝细胞癌 细胞凋亡 体外培养 |
INDUCTION OF APOPTOSIS OF HUMAN LIVER CARCINOMA CELLS BY TEA POLYPHENOLS |
| |
| Wu Qin,Fu Tiquan,Feng Chuanqian.INDUCTION OF APOPTOSIS OF HUMAN LIVER CARCINOMA CELLS BY TEA POLYPHENOLS[J].Medical Journal of Chinese People's Liberation Army,2001,26(2):110-112,F004. |
| |
| Authors: | Wu Qin Fu Tiquan Feng Chuanqian |
| |
| Institution: | Wu Qin,Fu Tiquan,Feng Chuanqian.The 302nd Hospital of PLA,Beijing 100039 |
| |
| Abstract: | Tea polyphenols were tried to induce apoptosis of human cultured hepatocellular carcinoma cell line HepG-Ⅱ. MTT assay, DNA agarose gel electrophoresis, transmission electron microscopy , fluorescence decoration and DNA end labeling method (Tunel) were used to identify apoptosis. Having been treated by tea polyphenols in 250μg/ml , HepG-Ⅱcell apoptosis was induced. The induction of apoptosis was the dose dependent. Chromatin condensation, apoptotic body formation, fluorescence of yellow green and pellet were observed. Agarose gel electrophoresis analysis revealed DNA cleavages( DNA ladder). The results indicated that tea polyphenole could induce apoptosis of cultured human hepatocellular carcinoma cell line HepG-Ⅱ. It suggested that tea polyphenols could be used to treat human liver carcinoma. |
| |
| Keywords: | tea polyphenols carcinoma hepatocellular apoptosis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
