独活对大鼠胶原诱导性关节炎的治疗作用

目的:观察不同剂量独活对胶原诱导性关节炎的治疗作用.方法:选择牛Ⅱ型胶原为免疫原,建立胶原诱导性关节炎模型,选取30只造模成功的大鼠随机分为5组:独活组[按顺序再分为低剂量(1.5 g· kg-1)组、中剂量(4.5 g· kg-1)组、大剂量(15 g· kg-1)组]和甲胺喋呤(B)组、生理盐水(C)组.采用关节炎指数评分对大鼠关节炎程度进行评分；采用酶联免疫吸附法测定各组大鼠血清基质金属蛋白酶-3、血管内皮生长因子,并评估可能的肝肾及胃黏膜副反应.结果:AI评分比较,中、小剂量独活组与生理盐水组差异无统计学意义；大剂量独活组小于生理盐水组(P＜0.05),独活各剂量组差异无统计学意义；甲胺喋呤组均小于生理盐水组、小、中剂量独活组(分别P＜0.01、P＜0.05、P＜0.05),而与大剂量独活组比较差异无统计学意义.血清血管内皮生长因子比较,独活各剂量组差异无统计学意义,但均显著低于生理盐水组(按大、中、小分别P＜0.01、P＜0.05、P＜0.05)；甲胺喋呤组显著低于生理盐水组及小剂量独活组(分别P＜0.01、P＜0.05),但与中、大剂量独活组比较差异无统计学意义.血清基质金属蛋白酶-3比较,各独活剂量组比较差异无统计学意义；独活小剂量组与生理盐水组差异无统计学意义；独活中、大剂量组均低于生理盐水组(均P＜0.05)；甲胺喋呤组低于生理盐水组及小剂量独活组(均P＜0.01),但与中、大剂量独活组比较差异无统计学意义.各组肝功、肾功、血常规及胃病理检查均未见明显异常.结论:①独活大剂量能减轻胶原诱导性关节炎炎症,而中低剂量则无此作用；②独活中大剂量能明显降低血清血管内皮生长因子、基质金属蛋白酶-3水平；③独活大剂量在短期动物实验中无肝肾功能、血液、胃组织损害.

Effect of Heracleum on collagen induced arthritis in rats

Objective:To observe the therapeutic effectiveness of different doses Heracleum on collagen induced arthritis(CIA) in rats.Methods:CIA rat model was established by subcutaneouly injecting bovine nasal cartilage II collagen.30 CIA Rats were randomly divided into three Heracleum(low,medium and high dose,A1 and A2 and A3) treated groups,MTX(Methotrexate,B) treated group,and control(C) group.The arthritic index(AI) of all rats were compared separately,the serum level of MMP-3,VEGF,AST,ALT,BUN,Cr were assayed,and the histology of gastric mucosal were examination.Result:In AI score,there was no difference between groupA2,groupA1 and groupC;that in groupA3 were less than GroupB(P<0.05,P<0.01),there was no statistical difference in Heracleum groups.In the serum level of VEGF,all the group A1 and A2 and A3 and B were less than the groupC(P<0.05,P<0.05,P<0.01,P<0.01),but the differences of groupA2 and groupA3 and groupB were not statistically significant.In the serum level of MMP-3,all the groupA2 and A3 and B were less than the groupC(P<0.05,P<0.05,P<0.01),but the differences of groupA2 and groupA3 and groupB were not statistically significant.In the serum level of AST,ALT,BUN,Cr and the histology of gastric mucosal,all groups showed no obvious abnormalities.Conclusion:①High dose of Heracleum can reduce the inflammation of CIA rats,while the low dose does not have this effect.②Medium and high dose of Heracleum can significantly reduce the serum level of VEGF and MMP-3.③For High dose of Heracleum,there are no damage of hepatic and renal function,blood and stomach in the short-term animal studies.