OSAHS患者腭咽组织血管内皮生长因子水平与微血管密度相关性分析

目的 探讨阻塞性睡眠呼吸暂停低通气综合征(OSAHS)患者腭咽组织中血管内皮生长因子(VEGF)水平与微血管密度(MVD)的相关性.方法 选择经多导睡眠监测仪(PSG)确诊的40例OSAHS患者组织标本(其中轻度7例,中度12例,重度21例),其中男性36例,女性4例；年龄29～62岁.6例无鼾症患者的软腭组织作为对照组,其中男性5例,女性1例；年龄18-58岁.采用苏木精-伊红(HE)染色法染色腭咽部组织,用光学显微镜观察其病理组织学改变,酶联免疫吸附分析检测血浆、腭咽组织匀浆中VEGF的含量,免疫组织化学技术检测MVD的表达情况.结果 酶联免疫吸附分析显示,OSAHS患者血浆及腭咽组织匀浆中VEGF水平均明显高于对照组,差异有显著统计学意义(P＜0.01)；中、重度OSA HS组与对照组比较,MVD表达差异均有显著统计学意义(P＜0.01),轻度组与对照组比较差异无统计学意义(P＞0.05)；组织匀浆中VEGF水平与MVD呈正相关(P＜0.01).结论 OSAHS患者腭咽部存在新生血管生成,与缺氧程度有关,VEGF在其发生发展过程中可能起到重要作用.

Relationship between vascular endothelial growth factor level and microvessel density in pharyngeal tissues of the patients with obstructive sleep apnea hypopnea syndrome

Objective To study the relationship between vascular endothelial growth factor（VEGF） level and microvessel density （MVD） in pharyngeal tissues of the patient with obstructive sleep apnea hypopnea syndrome （OSAHS）. Methods A total of 40 patients with OSAHS confirmed by polysomnogram（PSG） were enrolled（male 36, female 4, aged 29 - 62 years old）, included 7 with mild symptom, 12 with moderate symptom and 21 with severe symptom. Six healthy subjects were enrolled as controls（male 5, female 1, aged 18 - 58 years old）. The pharyngeal tissues were observed under microscope after hematoxylin-eosin （HE） staining. The levels of VEGF in plasma and pharyngeal tissues were tested by enzyme linked immunosorbent assay （ELISA）. Immunohistoehemistry on paraffin sections was performed with anti-human factor antibody to study MVD. Results The levels of VEGF in the plasma and tissue h0mogenate of OSAHS patients were higher than those of the control group（P 〈 0.01 ）. The MVD of moderate and severe OSAHS patients were higher than those of the control group（P 〈 0.01）, and MVD of mild OSAHS patients was not significantly different from that of the control group （P 〉 0.05）. The level of VEGF in tissue homogenate showed positive correlation with MVD（P 〈 0.01）. Conclusion It is demonstrated that angiogenesis has important relationship with hypoxia degree in patients with OSAHS, and VEGF may play a crucial role in its development.