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| 光谱法鉴定黑海参中的多糖成分 | |
| 引用本文： | 李熙灿,曾和平,郑雨,杜少辉,黄玲,陈东风.光谱法鉴定黑海参中的多糖成分[J].中国药房,2005,16(13):1012-1014. |
| 作者姓名： | 李熙灿 曾和平 郑雨 杜少辉 黄玲 陈东风 |
| 作者单位： | 1. 广州中医药大学,广州市,510405 2. 华南理工大学化学学院,广州市,510641 3. 广东汕头市中心医院,汕头市,515000 |
| 基金项目： | 国家自然科学基金项目(30271677，30371837) |
| 摘 要： | 目的:建立以光谱法鉴定黑海参中多糖成分的方法。方法:采用电泳法测定黑海参中多糖纯度,并用红外分光光度法、紫外分光光度法、核磁共振波谱法对其红外吸收光谱、紫外吸收光谱、核磁共振碳谱、核磁共振氢谱进行结构鉴定。结果:电泳后凝胶带呈蓝色,光谱检测结果可相互解释。结论:黑海参中存在均一组分的多糖成分,且为一种含硫酸酯基的酸性多糖,但不含糖肽;α-构型,存在α-吡喃葡萄糖,分子中的羟基形成分子内氢键。 |
| 关 键 词： | 黑海参 光谱 鉴定 多糖成分 |
| 文章编号： | 1001-0408(2005)13-1012-03 |
| 修稿时间： | 2005年3月21日 |
Identify Polysaccharide in Holothuria atra Jeager by Spectroscopy |
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| LI Xican,DU Shaohui,HUANG Ling,CHEN Dongfeng,Zeng Heping,ZHENG Yu.Identify Polysaccharide in Holothuria atra Jeager by Spectroscopy[J].China Pharmacy,2005,16(13):1012-1014. | |
| Authors: | LI Xican DU Shaohui HUANG Ling CHEN Dongfeng Zeng Heping ZHENG Yu |
| Abstract: | OBJECTIVE:To establish the method to identify the polysaccharide element in Holothuria atra Jeager by spectroscopy.METHODS:The purity of polysaccharide in Holothuria atra Jeager was determined by electrophoretic method;the infrared absorption spectrum,ultraviolet absorption spectrum,carbon spectra and hydrogen spectra of NMR were identified in structure by infrared spectrophotometry,ultraviolet spectrophotometry and NMR spectrometry respectively.RESULTS:The gel band after electrophoresis took blue color and the results of spectrum determination accountable for each other.CONCLUSION:Uniformly constituent of polysaccharose was found in the Holothuria atra Jeager,which was an acidic polysaccharose with vitriolic esterfunction,without glucopeptide;α-configuration,α-glucopyranose was found and the intramolecular hydrogen bond was formed by hydroxide radical. |
| Keywords: | Holothuria atra Jeager Spectrosopy Identification Polysaccharide |
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