臌胀片抗大鼠肝纤维化作用的实验研究

目的：观察臌胀片抗大鼠肝纤维化的作用，并探讨其机制。方法：将200～220g雌性大鼠50只，随机分为：正常空白对照组(简称对照组，10只)，病理模型对照组(简称模型组，10只)，臌胀片小剂量治疗组(简称小剂量组，10只)，臌胀片大剂量治疗组(简称大剂量组，10只)，鳖甲煎丸阳性对照组(简称鳖甲组，10只)。观察治疗8周后大鼠的相关血清酶类、肝纤维化标志物、氧自由基及肝组织病理学等变化。结果：(1)与模型组比较，大、小剂量组治疗后血清丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)、γ—谷氨酰转肽酶(GGT)、碱性磷酸酶(AKP)均有改善，大剂量组效果更为显著；(2)与对照组比较，大、小剂量组对血清总蛋白(TP)作用无明显差异，对白蛋白(ALB)有升高作用；(3)与对照组比较，大、小剂量组对血清透明质酸(HA)、层粘蛋白(LN)、Ⅳ型胶原(Ⅳ—C)、Ⅲ型前胶原(PCⅢ)均有降低的作用，大剂量组尤为显著；(4)大、小剂量组可以减少丙二醛(MDA)、谷胱甘肽过氧化酶(GSH—Px)和一氧化氮(NO)含量，然而在增加超氧化物歧化酶(SOD)水平方面，与鳖甲组比较，差异无显著性；(5)大、小剂量组具有明显抗纤维化的作用，并优于鳖甲组。结论：臌胀片在对抗大鼠肝纤维化的发生、发展过程中，能够保护肝细胞，抑制胶原蛋白的合成并促进其降解，其作用机制与对抗氧化损伤作用有关。

Experimental Study on the Anti-Liver Fibrosis Effect of Guzhang Tablet in Rats

OBJECTIVE: To observe the anti-liver-fibrosis effect of Guzhang Tablet (GZT) and to explore its mechanism. METHODS: Fifty female rats, weighing 200-220 g were randomly divided into the blank control group, the model group, the small dosage GZT group, the large dosage GZT group and the Biejiajian Pill (BJJP) group, 10 rats in each group. The changes of related serum enzymes, liver-fibrosis marker, oxygen free radical and liver tissue pathology were observed after 8 weeks of treatment. RESULTS: (1) Compared with the model group, the serum levels of alanine transaminase (ALT), aspartate aminotransferase (AST), glutamyltranspetidase (GGT), alkaline phosphatase (AKP) were improved in the GZT groups, better effect was got in the large dosage GZT group. (2) Compared with the blank control group, GZT showed no effect on serum total protein (TP) but with raising albumin (ALB) effect. (3) Compared with blank control group, the levels of hyaluronidase (HA), laminin (LN), collagen type IV (IV-C) and procollagen type III (PC III) in both GZT groups were lower, especially in the large dosage GZT group. (4) Small and large dosage of GZT showed effect in reducing malonyldialdehyde (MDA), Glutathione-peroxidose(GSH-Px) and nitric oxide (NO) content, but the effect on increasing superoxide dismutase (SOD) similar to that of BJJP with no significant different. (5) GZT, both small and large dosage, had obvious anti-liver fibrosis action, which was superior to that of BJJP. CONCLUSION: In the genetic and developing processes of liver fibrosis in rats, GZT could protect the liver cells, inhibit the synthesis and reduce the content of collagens, the mechanism might be related with its action in antagonizing peroxidation injury, indicating that GZT could effectively prevent liver fibrosis formation.