| 黄体酮促进培养乳腺癌细胞的增殖和迁移 |
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| 引用本文： | 赵嘉佳,王辛,霍中军,罗深秋,熊静波. 黄体酮促进培养乳腺癌细胞的增殖和迁移[J]. 南方医科大学学报, 2010, 30(3): 443 |
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| 作者姓名： | 赵嘉佳 王辛 霍中军 罗深秋 熊静波 |
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| 作者单位： | 南方医科大学细胞生物学教研室,广东,广州,510515;南方医科大学病理学教研室,广东,广州,510515 |
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| 基金项目： | 国家自然科学基金(30470673);;广东省自然科学基金(4020364);;教育部留学回国人员启动基金(教外司留[2004]176号) |
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| 摘 要： | 目的研究黄体酮对乳腺癌细胞增殖和迁移的影响。方法100nM黄体酮刺激黄体酮受体阳性的乳腺癌细胞株T-47D和MCF-748小时后,用MTT方法检测细胞增殖,用细胞划痕愈合实验检测细胞迁移,用Western blotting检测细胞内E-Cadherin的表达。结果黄体酮刺激MCF-7和T-47D细胞的增殖和迁移;并且可以下调MCF-7和T-47DE-Cadherin的表达。结论黄体酮能够促进培养乳腺癌细胞的增殖和迁移,提示抗黄体酮治疗可能有临床意义。
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| 关 键 词： | 黄体酮 乳腺癌 细胞增殖 细胞迁移 |
Progesterone promotes the proliferation and migration of cultured breast cancer cells |
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| ZHAO Jia-jia,WANG Xin,HUO Zhong-jun,LUO Shen-qiu,XIONG Jing-bo. Progesterone promotes the proliferation and migration of cultured breast cancer cells[J]. Journal of Southern Medical University, 2010, 30(3): 443 |
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| Authors: | ZHAO Jia-jia WANG Xin HUO Zhong-jun LUO Shen-qiu XIONG Jing-bo |
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| Affiliation: | ZHAO Jia-jia1,WANG Xin2,HUO Zhong-jun1,LUO Shen-qiu1,XIONG Jing-bo1 1Department of Cell Biology,2Department of Pathology,Southern Medical University,Guangzhou 510515,China |
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| Abstract: | Objective To investigate the effects of progesterone on the growth and migration of breast cancer cells. Methods MCF-7 and T-47D cells were cultured in DMEM and stimulated with 100 nmol/L progesterone for 48 h, and the cell proliferation was evaluated by MTT assay, cell migration by wound-healing assay and E-catherin expression by Western blotting. Results Progesterone stimulated the cell proliferation and migration and down-regulated the expression of E-catherin in both MCF-7 and T-47D cells. Conclusions P... |
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| Keywords: | progesterone breast cancer cell proliferation cell migration |
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