| 甲型H1N1（2009）流感流行后流感样病例呼吸道病原检测分析 |
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| 引用本文： | 单云峰,葛以跃,崔仑标,赵康辰,单军,戚宇华,崔岚,秦圆方,祁贤,霍翔.甲型H1N1（2009）流感流行后流感样病例呼吸道病原检测分析[J].江苏预防医学,2011,22(3):23-26. |
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| 作者姓名： | 单云峰 葛以跃 崔仑标 赵康辰 单军 戚宇华 崔岚 秦圆方 祁贤 霍翔 |
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| 作者单位： | 江苏省疾病预防控制中心,江苏,南京,210009 |
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| 基金项目： | 国家艾滋病、病毒性肝炎等重大传染病防治专项基金 |
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| 摘 要： | 目的：了解甲型H1N1（2009）流感流行后的流感样病例（ILI）中,除流感病毒外的相关病原分布,为ILI患者临床治疗、流行病学调查提供参考。方法：收集流感监测哨点医院393份ILI咽拭子标本提取核酸,应用实时荧光PCR方法进行20种相关病原比对检测,分析各病原的阳性率与构成比。结果：检测出阳性咽拭子标本51例,分属13种共计64株病原体,其中阳性率较高的为肺炎链球菌、流感嗜血杆菌、冠状病毒。另有13例为两种病原体混合感染,占阳性标本数的25.49%。结论：ILI的病原构成比较复杂,且存在混合感染,临床治疗与流行病学调查均应引起重视。
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| 关 键 词： | 流感样病例 病原体 实时荧光PCR 混合感染 |
Respiratory pathogens Detection of influenza-like cases after influenza pandemic of Influenza A H1N1 in 2009 |
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| SHAN Yun-feng,GE Yi-yue,CUI Lun-biao,ZHAO Kang-chen,SHAN Jun,QI Yu-hua,CUI Lan,QIN Yuan-fang,QI Xian,HUO Xiang.Respiratory pathogens Detection of influenza-like cases after influenza pandemic of Influenza A H1N1 in 2009[J].Jiangsu Journal of Preventive Medicine,2011,22(3):23-26. |
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| Authors: | SHAN Yun-feng GE Yi-yue CUI Lun-biao ZHAO Kang-chen SHAN Jun QI Yu-hua CUI Lan QIN Yuan-fang QI Xian HUO Xiang |
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| Institution: | (Jiangsu Province Center for Disease Prevention and Control,Nanjing 210009,China) |
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| Abstract: | Objective:To detect the pathogens expect influenza virus in the specimens from the tracheal aspirate of the patients with influenza-like illness(IL I) after the influenza A H1N1 pandemic in 2009,and to characterize the etiology spectrum which could be a useful reference for clinical therapy and epidemic surveillance.Methods: RNA/DNAs were extracted from 393 throat swab specimens of the patients with ILI.20 species of pathogens were comparison detected by real-time PCR,and the positive rate of each pathogen ... |
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| Keywords: | influenza-like illness pathogen real-time fluorescent PCR mixed infections |
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