前列腺素E_2、酵母多糖及雷公藤红素对大鼠腹腔细胞内cAMP水平的调节

PGE_2增加大鼠腹腔细胞内cAMP含量,其增加呈浓度依赖关系。蛋白胨刺激的大鼠,其细胞对PGE2反应更敏感。酵母多糖明显增加细胞内cAMP,于0.5 mg/ml时,上清液中cAMP亦有明显增加,同时增敏细胞对PGE_2的反应性。雷公藤红素(tripte-rine)1.0μg/ml降低cAMP含量,且明显抑制细胞对PGE_2及酵母多糖的反应性,部分解释了雷公藤红素的抗炎机理。

Regulation of PGE2 , zymosan and tripterine on cAMP level of rat peritoneal cells

PGE2 (0.03-30.0 μM) increased cAMP level in rat peritoneal cells in a dose dependent fashion. Zymosan significantly increased cAMP level in rat peritoneal cells but not in supernatants except with high concentration of zymosan.Peritoneal cells which were harvested from rats stimulated with proteose peptone responded to PGE2 more sensitively than rest cells. When combined with zymosan, rat peritoneal cells responded to PGE2 by anelevated and prolonged production of cAMP. PGE2 6μM raised cAMP to 49.4?.8 pmol/107 cells, zymosan 0.5 mg/ml alone increased cAMP content to 37.4?.9 pmol/ 107 cells. However, combined PGE2 6 μM and zymosan 0.5 mg/ml for 10 min elevated cAMP level to 116?4 pmol. So was the elevative pattern of cAMP when incubated the cells for 30 and 60 min. This suggests that the stimulant induced enhancement of PGE2 sensitivity may be a mechanism that selectively regulates macrophage function under physiologic conditions.Tripterine is one of the active components isolated from Tripterygium wilfordii Hook. In this study, it has been shown that 0.1-1.0μg/ml of tripterine reduced cAMP level of rat peritoneal cells markedly. It also inhibited the response of rat peritoneal cells to PGE2 and zymosan. In view ofthe important roles of PGs in inflammatory process, the effect of tripterine on cell response to PGE2 may give a partial explanation of its anti-inflammatory action.