反相高效液相色谱法测定当归拈痛丸中黄芩苷含量

目的建立当归拈痛丸中黄芩苷的含量测定方法。方法采用反相高效液相色谱法，色谱柱为C18柱（200mm×4．6mm，5μm），甲醇-0．2％磷酸溶液（45：55）为流动相，流速为1．0mL／min，检测波长为278nm，柱温35℃。结果黄芩苷选样量在0．06084～1．8252μg范围内与峰面积线性关系良好，r=1.00，平均加样回收率为97．0％，RSD为1．1％（n=9）。结论反相高效液相色谱法灵敏、简便、准确、重现性好，适用于当归拈痛丸的质量控制。

Determination of Baicalin in Danggui Niantong Pills by HPLC

Objective To establish a RP- HPLC method for the determination of baicalin in Danggui Niantong Pills. Methods RP- HPLC method was adopted and the C18 column （200 mm×4.6 mm,5 μm） was used at 35 ℃. The mobile phase was methanol-0. 2% phosphoric acid （45：55） with the flow rate of 1.0 mL/min. The detection wavelength was at 278 nm and the column temperature was 35℃. Results The calibration curve of baicalin was linear in the range of 0. 060 84 - 1. 825 2 μg. The average recovery rate was 97.0% , RSD = 1.1% （n =9）. Conclusion The method is highly sensitive, quick and accurate with good repeatability. It can be used for the quality control of Danggui Niantong Pills.