Articular chondroprogenitors in platelet rich plasma for treatment of osteoarthritis and osteochondral defects in a rabbit knee model |
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| Institution: | 1. Department of Physiology, Christian Medical College, Vellore 632002, India;2. Centre for Stem Cell Research, Christian Medical College, Vellore 632002, India;3. Department of Pathology, Smt NHL Municipal Medical College, Ahmedabad 380006, India;4. Department of Histology and Embryology Campus, School of Medicine, Akdeniz University, Antalya 07070, Turkey;5. Department of Biochemistry, Christian Medical College, Vellore 632002, India;6. Department of Orthopaedics, Royal Darwin Hospital, 105 Rocklands Drive, Tiwi, NT 0810, Australia;1. Department of Molecular Medicine Arthritis Research, The Scripps Research Institute, CA, USA;2. Department of Orthopaedic Surgery, Faculty of Medicine, The University of Tokyo, Tokyo, Japan;3. Department of Orthopaedic Biomaterial Science, Osaka University Graduate School of Medicine, Osaka, Japan;4. Department of Information Systems, Faculty of Engineering, Saitama Institute of Technology, Saitama, Japan;5. Department of Orthopaedic Surgery, Osaka Medical Center, Osaka, Japan;6. Shiley Center for Orthopaedic Research and Education at Scripps Clinic, CA, USA;1. Leeds Orthopaedic & Trauma Sciences, School of Medicine, University of Leeds, United Kingdom;2. Bradford Royal Infirmary, United Kingdom;3. Institute for Transport Studies, University of Leeds, United Kingdom;4. Leeds Institute of Rheumatic and Musculoskeletal Medicine, University of Leeds, United Kingdom;1. Department of Physiology/Centre for Stem Cell Research, Christian Medical College, Vellore, 632002, India;2. Department of Biochemistry, Christian Medical College, Vellore, 632002, India;3. Division of Experimental Pathology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, 695 012, India;4. St. Isabel''s Hospital, Mylapore, Chennai, 600004, India;5. Department of Histology and Embryology Campus, School of Medicine, Akdeniz University, Antalya, 07070, Turkey;6. Department of Orthopaedics/Centre for Stem Cell Research, Christian Medical College, Vellore, 632004, India;1. Department of Surgery, Stanford University School of Medicine, Palo Alto, California, U.S.A;2. Orthopaedic Soft Tissue Research Program, Sports Medicine and Shoulder Service, and the Department of Biomechanics, Hospital for Special Surgery, New York, New York, U.S.A;3. Department of Orthopaedic Surgery and Traumatology, University of Bern, Insel Hospital, Bern, Switzerland;4. Department of Orthopaedic Surgery, University at Albany–State University of New York, Albany, New York, U.S.A;5. Department of Orthopedic Surgery, University of São Paulo, São Paulo, Brazil |
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| Abstract: | BackgroundArticular chondroprogenitors are a promising contender for cartilage repair due to their inherent nature which stands primed for chondrogenesis and minimal hypertrophic preponderance. Platelet rich plasma (PRP) has been extensively used for treating cartilage defects and osteoarthritis (OA), due to its chondro-inductive properties and abundant pool of growth factors. The aim of this study was to assess the efficacy of chondroprogenitors injected with PRP versus PRP alone in the healing of experimentally created early OA and osteochondral defects (OCD) in a rabbit model.MethodsAdult New Zealand White male rabbits were used for cell and PRP isolation. Chondroprogenitors were isolated by fibronectin adhesion assay, labelled with iron oxide, characterized for surface markers, differential potential and expanded. PRP was isolated by double spin centrifugation using a TriCell kit. Study groups included (a) Monosodium iodoacetate induced early OA and (b) critical OCD. Following intervention (test arm: PRP+ chondroprogenitors and control arm: PRP), assessment was performed at 6- and 12-weeks which included histopathological examination and scoring (OARSI and Modified Wakitani score), immunohistochemistry analysis (Collagen type II and X) and synovial fluid S100A12 levels.Results and conclusionComparable, evident healing was noticed in both test and control arms when the OA group samples were assessed at both time points. In the OCD group, PRP alone exhibited significantly better results than the test arm, although repair was notable in both interventions. Further evaluation of chondroprogenitors is required to assess their role as a standalone therapy and in combination with PRP to further cartilage regeneration. |
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| Keywords: | Chondroprogenitors Osteoarthritis Osteochondral defects Platelet rich plasma Articular cartilage |
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