Lead (Pb2+)-induced calcium oxalate crystallization ex vivo is ameliorated via inositol 1,4,5-trisphosphate receptor (InsP3R) knockdown in a Drosophila melanogaster model of nephrolithiasis |
| |
| Institution: | 1. Laboratory of Environmental Chemistry and Toxicology, Department of Environmental Health Science, IRCCS-Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy;2. Interdisciplinary Center for Nanotoxicity, Department of Chemistry, Physics, and Atmospheric Sciences, Jackson State University, Jackson, MS, USA;3. Department of Informatics, Postdoctoral Institute for Computational Studies, Enfield, NH, USA;4. The Ronin Institute of Independent Scholarship, Montclair, NJ, USA;1. Graduate School of Global Environmental Studies, Kyoto University, Japan;2. Graduate School of Engineering, Kyoto University, Japan;1. Aquatic Environment and Health Management Division, ICAR- Central Institute of Fisheries Education, Mumbai, 400 061, India;2. College of Fisheries, Central Agricultural University (I), Lembucherra, Tripura, 799210, India;1. Department of Environmental Science and Policy, University of Milan, via Celoria 26, 20133, Milan, Italy;2. Department of Animal Ecology I and BayCEER, University of Bayreuth, Universitaetsstraße 30, 95447, Bayreuth, Germany |
| |
| Abstract: | Nephrolithiasis causes severe pain and is a highly recurrent pathophysiological state. Calcium-containing stones, specifically calcium oxalate (CaOx), is the most common type accounting for approximately 75 % of stone composition. Genetic predisposition, gender, geographic region, diet, and low fluid intake all contribute to disease pathogenesis. However, exposure to environmental pollutants as a contribution to kidney stone formation remains insufficiently studied. Lead (Pb2+) is of particular interest as epidemiological data indicate that low-level exposure (BLL = 0.48–3.85 μM) confers a 35 % increased risk of developing CaOx nephrolithiasis. However, mechanisms underlying this association have yet to be elucidated. Drosophila melanogaster provide a useful genetic model where major molecular pathophysiological pathways can be efficiently studied. Malpighian tubules (MT) were isolated from either Wild-Type or InsP3R knockdown flies and treated with oxalate (5 mM) ± Pb2+ (2μM) for 1 h. Following exposure, MTs were imaged and crystals quantified. CaOx crystal number and total area were significantly increased (?5-fold) in Pb2+(pre-treatment) + oxalate-exposed MTs when compared to oxalate alone controls. However, CaOx crystal number and total crystal area in Pb2+ + oxalate-exposed InsP3R knockdown MTs were significantly decreased (?3-fold) indicating the role for principal cell-specific InsP3R-mediated Ca2+ mobilization as a mechanism for Pb2+-induced increases in CaOx crystallization inset model of nephrolithiasis. |
| |
| Keywords: | Lead poisoning Calcium oxalate nephrolithiasis Insect model |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
