Tissue-specific genotoxicity and antioxidant imbalance of titanium dioxide nanoparticles (NPTiO2) and inorganic lead (PbII) in a neotropical fish species |
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| Affiliation: | 1. Department of Genetics, Laboratory of Animal Cytogenetics and Environmental Mutagenesis, Federal University of Paraná (UFPR), Curitiba, Paraná, Brazil;2. Department of Pharmacology, Laboratory of Aquatic Toxicology, Federal University of Paraná (UFPR), Curitiba, Paraná, Brazil;3. Department of Chemistry, State University of Ponta Grossa (UEPG), Ponta Grossa, Paraná, Brazil;1. University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Centre of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Vodňany, Czech Republic;2. Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Faculty of Science, Palacký University in Olomouc, 17 listopadu 12, 771 46 Olomouc, Czech Republic;1. Laboratório de Toxicologia Celular, Departamento de Biologia Celular, Universidade Federal do Paraná, Caixa Postal 19031, CEP 81531-970, Curitiba, PR, Brazil;2. Programa de Pós-Graduação em Ecologia e Conservação, Setor de Ciências Biológicas, Universidade Federal do Paraná, Caixa Postal 19031, CEP 81531-980, Curitiba, Paraná, Brazil;3. Estação de Piscicultura Panamá, Est. Geral Bom Retiro, CEP 88490-000, Paulo Lopes - SC, Brazil;4. Instituto Oceanográfico da Universidade de São Paulo, Praça do Oceanográfico, 191, 05508-900, São Paulo, SP, Brazil;5. Centro de Estudos do Mar, Universidade Federal do Paraná, Caixa Postal 61, 83255-976, Pontal do Paraná, PR, Brazil;6. Departamento Acadêmico de Química e Biologia, Universidade Tecnológica Federal do Paraná, Curitiba, Paraná, Brazil;7. Núcleo de Estudos em Poluição e Ecotoxicologia Aquática, Universidade Estadual Paulista, São Vicente, São Paulo, Brazil;8. Departamento de Ciências do Mar, Universidade Federal de São Paulo, Santos, São Paulo, Brazil;9. Departamento de Física, Universidade Federal do Paraná, 81531-990, Curitiba, Paraná, Brazil;1. Ecology and Conservation Program Post-Graduation, Federal University of Paraná, Box 19031, 81530-990, Curitiba-PR, Brazil;2. Department of Genetics, Federal University of Paraná, Box 19031, 81530-990, Curitiba-PR, Brazil;3. Department of Pharmacology, Federal University of Paraná (UFPR), Box 19031, 81530-990, Curitiba-PR, Brazil;4. Department of Physiology, Federal University of Paraná, Box 19031, 81530-990, Curitiba-PR, Brazil;5. Institute of Biophysics Carlos Chagas Filho 21941-902, Ilha do Fundão, Rio de Janeiro, Rio de Janeiro, Brazil |
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| Abstract: | The aquatic environment is the major recipient of wastes containing nanoparticles and other contaminants. Titanium dioxide nanoparticles (NPTiO2) are one of the most produced and used nanoparticle worldwide. This study investigated the toxicity of NPTiO2, as well as the toxicity interaction between NPTiO2 and lead (Pb), in response to genetic and biochemical biomarkers using freshwater fish Rhamdia quelen, as an animal model. The results showed genotoxicity in blood and kidney tissues. No effect of NPTiO2 alone or in co-exposure with Pb on liver genotoxicity were observed. Alterations in the antioxidant hepatic enzymes activities, as well as alterations in glutathione levels indicated that NPTiO2 alone or in co-exposure with Pb can cause antioxidant imbalance. The lipid peroxidation was also raised after exposure to NPTiO2. In general, the results of this study indicated that both NPTiO2 alone and their co-exposure with Pb are capable of producing significant toxic effects in short-term exposure. |
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| Keywords: | Nanomaterials Metal Co-exposure Comet assay Piscine micronucleus test Biochemical biomarkers |
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