| 胶质瘤细胞系A172、U251、U87和SHG-44中HER2的表达及意义 |
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| 引用本文： | 张雷鸣,张剑宁,杨双武,董超,方丹东,李娟,陈晓燕. 胶质瘤细胞系A172、U251、U87和SHG-44中HER2的表达及意义[J]. 中华神经外科疾病研究杂志, 2011, 10(2): 151-153 |
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| 作者姓名： | 张雷鸣 张剑宁 杨双武 董超 方丹东 李娟 陈晓燕 |
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| 作者单位： | 第四军医大学西京脑科医院神经外科,陕西,西安,710032 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的 探讨人表皮生长因子受体2(HER2)在人神经胶质瘤细胞系A172、U251、U87和SHG-44中的表达情况.方法 采用Western blot 和流式细胞术(FCM)于蛋白质水平检测HER2在四种人胶质瘤细胞系中的表达.结果 Western blot检测出HER2在人胶质瘤细胞系A172中的表达量最高,U251次之,U87和SHG-44表达量较低.FCM检测结果显示HER2在A172、U251、U87和SHG-44中的阳性率分别为36.5%、21.5%、3.3%和3.5%.结论 人胶质瘤细胞系A172和U251中HER2的表达量较高,这两种细胞系可作为良好的细胞模型来研究HER2分子在胶质瘤中的作用机制以及以HER2为靶点的生物治疗等.
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| 关 键 词： | 胶质瘤 细胞系 |
Expression of HER2 in glioma cell lines A172, U251, U87 and SHG-44 and its significance |
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| ZHANG Leiming,ZHANG Jianning,YANG Shuangwu,DONG Chao,FANG Dandong,LI Juan,CHEN Xiaoyan. Expression of HER2 in glioma cell lines A172, U251, U87 and SHG-44 and its significance[J]. Chinese Journal of Neurosurgical Disease Research, 2011, 10(2): 151-153 |
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| Authors: | ZHANG Leiming ZHANG Jianning YANG Shuangwu DONG Chao FANG Dandong LI Juan CHEN Xiaoyan |
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| Affiliation: | Department of Neurosurgery,Xijing Institute of Clinical Neuroscience,Fourth Military Medical University,Xi＇an 710032,China |
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| Abstract: | Objective To investigate the expression of human epidermal growth factor receptor 2（HER2） in glioma cell lines A172,U251,U87 and SHG-44.Methods The expression of HER2 in human glioma cell lines A172,U251,U87 and SHG-44 was detected by Western blot and flow cytometry（FCM）.Results Western blot demonstrated that the expression of HER2 was the highest in A172 followed by U251,and lowest in the SHG-44 and U87.FCM analysis indicated that the positive expression rate of HER2 in A172,U251,U87 and SHG-44 cell lines were 36.5%,21.5%,3.3%,and 3.5%,respectively.Conclusion HER2 is highly-expressed in glioma cell lines A271 and U251,which can be used as an ideal cell model for the research of the role of HER2 in glioma and the specific therapies targeting HER2. |
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| Keywords: | HER2 |
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