一种新型大鼠门腔静脉转位模型的建立

目的探讨一种新型大鼠门腔静脉转位模型建立的可行性。方法大鼠40只，采用袖套法建立同种异体血管门腔静脉转位模型。于左肾静脉末端安装一袖套，将左肾静脉与门静脉残端借袖套连接，贴近左肾静脉上方结扎并剪断下腔静脉，将下腔静脉借同种异体血管及袖套与肠系膜上静脉连接。另取10只大鼠切除左肾后作为对照组。观察术后大鼠的体重、生化指标、核磁共振及其肝脏病理学变化。结果40只大鼠门腔静脉转位后近、远期存活良好，术后24h至2月存活率均为97．50％（39／40）。术后2周核磁共振检查显示血流通畅。与对照组比较，实验组大鼠术后2月白蛋白、谷丙转氨酶、碱性磷酸酶和总胆红素、体重等指标差异均无统计学意义。术后2月肝组织病理检查结果正常。结论采用同种异体血管借助袖套方法建立大鼠门腔静脉转位模型是可行的。

Establishment of a new portacaval transposition model

Objective To explore the feasibility of a new portacaval transposition model establish.Methods The allograft rat model of portacaval transposition was established by using vascular cuff way.At the end of the installation of a cuff of left renal vein,the left renal vein and the portal vein stump by cuff connected,affixed above the left renal vein ligation and cut the inferior vena cava,the inferior vena cava by allogeneic cuff and superior mesenteric vessels and venous connection.Another 10 rats served as co...