| 血小板活化因子对大鼠卵巢颗粒细胞E2分泌的影响及机制 |
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| 引用本文： | 张承玉,李海侠,孙卫.血小板活化因子对大鼠卵巢颗粒细胞E2分泌的影响及机制[J].放射免疫学杂志,2009,22(5):438-440. |
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| 作者姓名： | 张承玉 李海侠 孙卫 |
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| 作者单位： | 黑龙江省牡丹江医学院生理教研室,157011 |
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| 基金项目： | 黑龙江省教育厅科研基金资助项目，牡丹江医学院科研项目 |
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| 摘 要： | 目的：观察血小板活化因子（PAF）对体外培养的大鼠卵巢颗粒细胞E2分泌的影响,初步探讨其可能的作用机制。方法：原代细胞培养和放免技术。结果：PAF、FSH、forscolin、PMA都能明显刺激大鼠卵巢颗粒细胞E2的分泌（P〈0.05）,PAF对FSH、forscolin、PMA诱导的大鼠卵巢颗粒细胞E2分泌均有明显的增强作用（P〈0.05）。结论：PAF能明显促进体外培养的大鼠卵巢颗粒细胞E2的分泌,并且受FSH及PKA、PKC信号转导通路的调节。
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| 关 键 词： | 血小板活化因子 颗粒细胞 雌二醇 |
Effect of Platelet Activating Factor(PAF) on Rat Ovarian Granulosa Cell Secretion of E_2 |
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| Zhang Chenyu,Li Haixia,Sun Wei.Effect of Platelet Activating Factor(PAF) on Rat Ovarian Granulosa Cell Secretion of E_2[J].Journal of Radioimmanology,2009,22(5):438-440. |
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| Authors: | Zhang Chenyu Li Haixia Sun Wei |
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| Institution: | Zhang Chenyu,Li Haixia,Sun WeiDepartment of Physiology,Mudanjiang Medical College,Heilongjiang(157011),China |
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| Abstract: | Objective To study the possible mechanism of augmentation of rat ovarian granulosa cell secretion of E2 in vitro by PAF.Methods Rat ovarian granulosa cells were recruited and cultured under different condifions： ① no stimulant added ② FSH 1μg/ml added ③ PAF 1μg/ml added ④ forscolin 100ng/ml added ⑤ phorbol-12-myristate 13-acetate（PMA） 100ng/ml added.⑥ PAF and FSH combined ⑦ PAF and forscolin combined ⑧ PAF and PMA combined.The E2 concentrations in various cultures were determined with RIA.Results Each of the four stimulants PAF,FSH,forscolin,PMA could induce hypersecretion of E2 by rat ovarian granulosa cells（P〈0.05）.Combined PAF with the other three stimulants could furthar augment the hypersecretion（P〈0.01）.Conclusion PAF could stimulate the E2 secretion from cultured rat ovarian granulosa cells via pka and pkc signa transmission pathways. |
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| Keywords: | PAF granulose cells estradiol(E2) |
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