1,25-(OH)2D3在骨髓间质成骨细胞与血管内皮细胞联合培养中的价值

目的 研究兔骨髓间质成骨细胞（RMSO）与兔肾脏血管内皮细胞（RRVEC）共培养时，1，25-（OH）2D3对细胞活性及碱性磷酸酶（ALP）活性、骨钙素（OCN）含量的影响。方法以相同总细胞数接种24孑L细胞培养板，1，25-（OH）2D3作用5d后，MTT比色法测定细胞活性。以相同RMSO细胞数接种96孑L细胞培养板，分别于1，25-（OH）2D3作用3、5、12d时，测定细胞ALP活性、OCN含量。结果 各检测时间点1，25-（OH）2D3作用组及与RRVEC共培养组细胞活性、ALP活性和OCN含量均较RMSO单独培养增强（P〈0．05）。1，25-（OH）2D3作用于RMSO、RRVEC共培养时，在各检测时间点，细胞活性、ALP活性、OCN含量均进一步增强（P〈0．05）。结论 1，25-（OH）2D3单独应用可促使RMSO成熟，1，25-（0H）2D3作用于RMSO、RRVEC共培养时，细胞活性、RMSO细胞分化及成骨能力均进一步增强。

Role of 1,25-dihydroxyvitamin D3 in co-culture of rabbit marrow mesenchymal stem cell and endothelial cell

Objective To evaluate the effect of 1,25-dihydroxyvitamin D_31,25-(OH)_2D_3]on co-culture of rabbit marrow mesenchymal stem cells(MSC) and renal endothelial cells(EC).Methods(With) same number of total cell in 24-well tissue culture plate,cell activity was evaluated on the 5th day after use of 1,25-(OH)_2D_3 and with same number of MSC in 96-well tissue culture plate,alkaline phosphatase(ALP) activity and osteocalein(OCN) production were detected on the 3rd,5th,and 12th day after 1,25-(OH)_2D_3 application.Results Application of 1,25-(OH)_2D_3 had the same effect on MSC as co-culture of EC,which all made the cell activity,ALP,OCN increased.When 1,25-(OH)_2D_3 applied to co-culture of MSC and EC,all the measurements increased farther.Conclusion(1,25-(OH)_2D_3) can accelerate the differentiation of MSC and when it is applied to co-culture of MSC and EC,the bone forming ability of MSC is increased further.