从血液中提取DNA方法探讨

目的:研究从血液中提取DNA的方法以应用。方法:静脉抽取血样,分别抗凝或不加抗凝处理后,提取DNA,通过电泳和PCR进行检测。结果:凝血和抗凝血的DNA产量和纯度分别是(40.2±8.86)mgDNA/L血液和(1.87±0.11)mgDNA/L,(39.1±10.2)mgDNA/L血液和(1.92±0.12)mgDNA/L。所有样本的DNA分子量都很高,从两者的DNA样本中能很容易地扩增出t-PA基因的第8内含子区Alu等位基因二态性,因此所提取的DNA是完整可靠的。结论:该方法能快速、简单、有效、无毒地从新鲜血液和凝血中提取DNA,适合于临床检测和分子生物学研究。

DNA Isolation by Simple Rapid and Innocuous Method from Blood Useful in Clinical Molecular Testing

Objective To study the method of DNA isolation from blood for application. Methods DNA was isolated and determinated by agarose gel electrophoesis and PCR from antiagglutinating and agglutinating blood samples that were withdrawn from auricular veins. Results The yields and purity of DNA isolated from clotted blood and antiagglutinating blood were (40.2 ± 8.86) mg DNA/L blood and (1.87 ± 0.11),(39.1 ± 10.2) mg DNA/L blood and (1.92± 0.12). The DNA that we isolated from all samples has high molecular weight and by PCR the dimorphism of Alu alleles of the 8th intron of t-PA was easy to be obtained, so it was complete and reliable. Conclusion The results showed this method is rapid, easy, efficient and innocuous for isolation of DNA from clotted and fresh blood and it is suit for clinical testing and molecular biology study.