实时荧光定量PCR检测胃癌组织Twist基因的结果分析

目的分析实时荧光定量PCR检测胃癌组织Twist基因的临床意义。方法用实时荧光定量PCR的方法检测正常胃黏膜、胃癌原发灶、胃癌转移灶组织中TwistmRNA的表达含量。并以Twist基因和18stRNA含量的比值作为评价Twist表达水平的指标，组间进行配对t检验。结果Twist／18stRNA（10g比值）正常组为0．139±0．003；原发组为0．304±0．046；转移组为0．654±0．015。原发性胃癌组织及转移组织中Twist基因表达水平与正常胃黏膜组织比较，差异有统计学意义（P〈0．05）；转移组与原发组比较，差异有统计学意义（P〈0．05）。结论Twist基因在正常胃黏膜组织中表达量较低，在胃癌及其转移组织中表达水平较高，而且Twist基因的表达水平与胃癌转移程度存在一定相关性。可以辅助诊断和观察胃癌术后疗效。

Results Analysis of Real-time Quantitative PCR in Detection of Twist Gene of Gastric Cancer Tissue

[Objective]To analyze the clinical value of real-time quantitative PCR in detection of Twist gene of gastric cancer tissue. [Methods] Real-time quantitative PCR method was used to detection the expression level of Twist mRNA in normal gastric mucosa, primary foci of gastric cancer, Metastasis of gastric cancer tissues. The ratio of Twist gene content to 18s tRNA genes content was taken as the evaluation indicators for expression level of Twist. Inter-group t-test was carried out. [ Results] Twist/18 s Trna of normal group was 0. 139 ± 0. 003 （ log rhtio） ; that of primary group was 0. 304 ±0. 046; that of metastasis group was 0. 65±0. 015. The expression levels of Twist levels in primary gastric cancer was significantly different from that in normal gastric mucosa （ P 〈0.05） ; the significant difference was also existed between metastatic gastric cancer and primary gastric cancer（ P 〈0. 05 ）. [ Conclusion] Twist gene expresses low level in normal gastric mucosa, but high levels in gastric cancer and metastasis issues. Twist gene expression levels relates with the metastatic gastric cancer which can assist clinical diagnosis and observation of curative effect of gastric cancer.