| 绞股蓝药材黄酮类成分HPLC指纹图谱研究 |
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| 引用本文： | 卢金清,陈黎,肖波,万威,喻樊,田耀平. 绞股蓝药材黄酮类成分HPLC指纹图谱研究[J]. 中药材, 2007, 30(8): 929-932 |
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| 作者姓名： | 卢金清 陈黎 肖波 万威 喻樊 田耀平 |
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| 作者单位： | 1. 湖北中医学院,湖北武汉,430061
2. 湖北中医学院,湖北武汉,430061;郧阳医学院附属太和医院,湖北十堰,442000 |
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| 摘 要： | 目的：建立绞股蓝药材黄酮类成分HPLC指纹图谱。方法：采用反相高效液相色谱法，色谱柱：TURNERKromasil^TM C18色谱柱（250×4．6mm，5μm）；流动相：0．4％磷酸水溶液-甲醇-乙腈（梯度洗脱）；流速：0．5ml／min；检测波长：360nm。结果：建立了绞股蓝药材黄酮类成分HPLC指纹图谱，标定了10个共有指纹特征峰，方法学考察符合指纹图谱技术要求。结论：方法稳定，可靠，精密度高，重复性好，可为绞股蓝药材质量标准的制定提供科学依据。
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| 关 键 词： | 绞股蓝 黄酮类成分 指纹图谱 |
| 文章编号： | 1001-4454（2007）08-0929-04 |
| 修稿时间： | 2006-12-14 |
Studies on the HPLC Fingerprint Spectrum of Flavonoids in Gynostemma pentaphyllum |
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| LU Jin-qing,CHEN Li,XIAO Bo,WAN Wei,YU Fan,TIAN Yao-ping. Studies on the HPLC Fingerprint Spectrum of Flavonoids in Gynostemma pentaphyllum[J]. Journal of Chinese medicinal materials, 2007, 30(8): 929-932 |
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| Authors: | LU Jin-qing CHEN Li XIAO Bo WAN Wei YU Fan TIAN Yao-ping |
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| Affiliation: | 1. Hubei College of Traditional Chinese Medicine, Wuhan 430061, China; 2. Yunyang Medical College Affiliated Taihe Hospital, Shiyan 442000, China |
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| Abstract: | OBJECTIVE: To establish the HPLC fingerprint spectrum of flavonoids from Gynostemma pentaphyllum. METHODS: The HPLC conditions with TURNER Kromasil C18 column (250 x 4. 6 mm, 5 microm) was used, the mobile phase consisted of 0.4% phosphoric acid-methanol-acetonitrile with gradient elution at flow rate of 0.5 ml/min and the detection wavelength was set at 360nm. RESULTS: Ten common characteristic peaks were taken as fingerprint peaks the presision and the retest are in accordance with the requirement. CONCLUSION: The method is stable, reliable, precision and provides a scientific basis for the quality standard of Gynostemma pentaphyllum. |
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| Keywords: | HPLC |
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