姜黄素对HepG-2细胞增殖与凋亡的影响

目的：检测姜黄素对肝癌HepG-2细胞增殖及细胞凋亡的作用，以及对核转录因子κB（NF-κB）表达的影响。方法：不同浓度（10，25，50，100 μmol/L）姜黄素处理肝癌HepG-2细胞不同时间后（16，24，48 h），采用CCK-8法检测细胞增殖抑制率；50 μmol/L的姜黄素处理HepG-2细胞24 h后，用流式细胞仪检测细胞凋亡情况；不同浓度（10，25，50，100 μmol/L）姜黄素处理肝癌HepG-2细胞24 h后，用Western blot方法检测NF-κB p65蛋白的表达。结果：姜黄素能明显抑制HepG-2细胞的增殖，并呈时间和浓度依赖性（均P<0.05）；姜黄素作用后，HepG-2细胞凋亡率较对照组明显增高（P<0.05）；姜黄素能浓度依赖性抑制地HepG-2细胞 NF-κB p65蛋白的表达。结论：姜黄素能抑制HepG-2细胞增殖并促进其凋亡，其机制可能系通过阻断NF-κB信号通路有关。

Effects of curcumin on proliferation and apoptosis of HepG-2 cells

Objective: To investigate the effect of curcumin on the proliferation and apoptosis, as well as on expression of nuclear factor κB (NF-κB) in human hepatocellular carcinoma HepG-2 cells. Methods: The inhibition rate of cell proliferation was determined by CCK-8 assay after HepG-2 cells were exposed to different concentrations of curcumin (10, 25, 50 and 100 μmol/L) for different time periods (16, 24 and 48 h). Cell apoptosis was detected by flow cytometry following exposure of HepG-2 cells to μmol/L curcumin for 24 h. The NF-κB p65 expression in HepG-2 cells was measured by Western blot analysis after treatment with different concentrations of curcumin (10, 25, 50 and 100 μmol/L) for 24 h. Results: Curcumin significantly inhibited the proliferation of HepG-2 cells in a time- and concentration-dependent manner (all P<0.05). The apoptosis rate of HepG-2 cells was significantly increased after curcumin treatment versus untreated HepG-2 cells (P<0.05). The NF-κB p65 expression in HepG-2 cells was down-regulated in a concentration-dependent manner after incubation with gradient concentrations of curcumin. Conclusion: Curcumin can inhibit the growth and promote apoptosis of HepG-2 cells, and the mechanism may be associated with its blockage of NF-κB pathway.