乳腺浸润性导管癌中CD74的表达及临床意义

目的:研究乳腺浸润性导管癌中CD74的表达及其临床意义.方法:用免疫组化技术检测45例乳腺浸润性导管癌组织中CD74,雌激素受体(ER),孕激素受体(PR)及c-erbB-2的表达,分析CD74表达与ER,PR及c-erbB-2表达及临床病理特征和预后的关系；观察乳腺癌MB-MDA-435细胞(高侵袭性),MCF-7细胞(低侵袭性)和转染CD74表达质粒的MCF-7细胞中CD74蛋白的表达,并用Transwell小室检测3种细胞的侵袭能力.结果:乳腺浸润性导管癌中,CD74的表达量与病理分级有关(P＜0.01),癌细胞分化愈差则CD74表达愈高；CD74在受体阳性组中的表达率明显低于受体阴性组(P＜0.01)；三阴乳腺癌中CD74的阳性表达率明显高于非三阴乳腺癌组(P＜0.01)；CD74阳性患者淋巴结转移率明显高于阴性表达者(P＜0.01)；CD74表达阳性与否与患者的5年生存率未见明显关系性.高侵袭性MB-MDA-435细胞CD74蛋白表达量明显高于低侵袭性MCF-7细胞；MCF-7细胞转染CD74表达质粒后侵袭能力明显增强.结论:CD74可能在乳腺癌的侵袭和淋巴结转移中起着重要作用,并有可能成为一种潜在的高侵袭性乳腺癌特别是三阴乳腺癌的标志物.

CD74 expression in invasive ductal carcinoma of breast and its clinical significance

Objective: To investigate CD74 expression in invasive ductal carcinoma (IDC) of the breast and its clinical significance.Methods: The CD74 expression along with the expressions of estrogen receptor (ER), progesterone receptor (PR) and c-erbB-2 in 45 IDC tissues were determined by immunohistochemical staining, and then the relations of CD74 expression with the expressions of ER, PR and c-erbB-2 as well as with the clinicopathologic profiles and prognosis of the patients were analyzed. CD74 expression in three breast cancer cells that were MB-MDA-435 cells (highly invasive), MCF-7 cells (low invasive) and CD74 expression plasmid transfected MCF-7 cells were observed, and then the invasion abilities of the three cells were tested by Transwell assay.Results: In IDC, CD74 expression level was significantly related to pathologic grade of IDC, which was the poorer the differentiation of cancer cells, the higher was the expression of CD74; the CD74 expression rate in receptor-positive group was significantly lower than that in receptor-negative group; the expression rate of CD74 in triple-negative breast cancer was significantly higher than that in non-triple-negative breast cancer; the rate of lymph node metastasis in CD74 positive patients was higher than that in CD74 negative cases; CD74 expression was not related to 5-year survival rate of patients. CD74 expression intensity in highly invasive MB-MDA-435 cells was significantly higher than that in low invasive MCF-7 cells, while the invasive ability of MCF-7 cells was increased after transfection with CD74 expression plasmid.Conclusion: CD74 may play an important role in invasion and lymph node metastasis of breast cancer, and it may also be a potential marker for high invasive breast cancer, especially the triple-negative breast cancer.