Chemical characterization of Escherichia coli K 1 capsular polysaccharide |
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| Authors: | M Mehl R Starke B Kissig R Grunow S Jahn G Schmidt R von Baehr |
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| Affiliation: | Institut für Medizinische Mikrobiologie des Bereiches Medizin, Humboldt-Universit?t zu Berlin. |
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| Abstract: | The exact chemical characterization of the prepared Escherichia coli K 1 capsule polysaccharide is necessary and a prerequisite for using this antigen as a screening antigen in the production of monoclonal anti-K 1-antibodies. The K 1-antigen, prepared by phenol-water-extraction, was analyzed by protein, RNA, and neuraminic acid determination. An addition, the antigen was subjected to elementary analysis, infrared- and 13C-NMR-spectroscopy, and gelchromatography. After testing the serological specificity, the K 1-antigen was identified as a high molecular polyneuraminic acid. |
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