毛细管电泳法测定小鼠组织中的反义核酸药物KS0604

目的:建立小鼠组织中KS0604的定量分析方法.方法:采用组织消化结合基于离子交换和反相分配原理的两步固相萃取法,并采用无胶筛分毛细管电泳技术测定小鼠组织中的KS0604.结果:小鼠肾组织中KS0604在2.0~2 000 μg·g-1范围内呈良好线性,最低定量限LOQ为2.0 μg·g-1;其他组织中KS0604在1.3~128 μg·g-1范围内呈良好线性,最低定量限LOQ为1.3 μg·g-1;r＞0.999.加样回收率试验RSD＜10.82%,精密度试验RSD＜2.3%,稳定性试验结果表明KS0604在小鼠组织中的稳定性良好.结论:该方法可用于定量检测小鼠组织中的KS0604.

Quantitative Determination of an Antisense Nucleic Acid Drug KS0604 in Mouse Tissues by CE

Objective:To establish a method for the quantitative determination of KS0604 in the mouse tissues.Method:A dual solid phase extraction（SPE） pretreatment method coupling with non-gel sieving capillary electrophoresis（NGCE） was established to determine the content of KS0604 in the tissues of mice.Result:The calibration curve of KS0604 was linear within the concentration range of 2.0-2 000μg ? g^-1 in kidney and 1.3-128μg ? g^-1 in the other mouse tissues with correlation coefficients（r） above 0.999. The LLOQ was 2.0μg ? g ^-1 in kidney and 1.3μg ? g ^-1 in the other mouse tissues.The RSD of the sample-adding recovery of KS0604 in mouse tissues was less than 10.82%and the precision RSD of the assay was below 2.3%.KS0604 was stable in mouse tissues. Conclusion:The combined method of solid-phase extraction and NGCE can be used in the detection and quantitative determination of KS0604 in mouse tissues.