EFFECT OF CHRONIC ETHANOL CONSUMPTION ON GLYCOSYLATION PROCESSES IN RAT LIVER MICROSOMES AND GOLGI APPARATUS |
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| Authors: | COTTALASSO, D. GAZZO, P. DAPINO, D. DOMENICOTTI, C. PRONZATO, M. A. TRAVERSO, N. BELLOCCHIO, A. NANNI, G. MARINARI, U. M. |
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| Affiliation: | Institute of General Pathology, School of Medicine, University of Genoa Italy |
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| Abstract: | Previous studies have demonstrated that acute ethanol intoxicationaffects various steps of protein glycosylation at the levelof rat liver endoplasmic reticulum and Golgi apparatus. Theaim of this investigation was to demonstrate whether chronicethanol intake can induce definitive changes of liver glycoproteinprocessing. Rats were given ethanol by liquid diet for 8 weeks.At the end of this period the triglyceride levels in liver homogenateand microsomes were significantly higher than in controls. Isolatedhepatocytes prelabelled with [3H]Na palmitate and [14C]glucosamineshowed a significant storage of the lipid and carbohydrate radioactivityin microsomes and Golgi apparatus and a significant impairmentof labelled glycolipoprotein secretion. Changes of the glycosylationsteps were observed both in endoplasmic reticulum and in Golgiapparatus: in the former the levels of dolichyl phosphate, whichis rate-limiting for the synthesis of glycoprotein, showed asignificant reduction; in the latter the activity of the mainenzymes responsible for the terminal glycosylation process wassignificantly decreased. These data suggest that an impairmentof glycoprotein maturation may be involved in the pathogenesisof liver injury induced by chronic ethanol intake. |
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