液氮冷冻联合Nd:YAG激光对兔耳静脉作用的效果观察

目的:观察液氮冷冻联合Nd:YAG激光照射对兔耳静脉的作用,评估该方法治疗静脉畸形的可行性。方法:将65只白兔随机分为4组。A、B、C为实验组,每组20只;D组为空白对照组,5只。以兔耳背中央静脉为实验模型,A组行液氮冷冻及Nd:YAG激光照射,B组行液氮冷冻,C组行Nd:YAG激光照射,D组不做处理。分别于实验处置后1、3、7、14、21 d对兔耳背中央静脉进行大体、光镜及电镜观察。结果:A组静脉血管内皮细胞、管壁平滑肌及构架结构均有明显损伤破坏,管腔内血栓形成,最终静脉闭锁。B、C组虽有血管内皮细胞损伤及血栓形成,但随后血栓逐渐溶解,血管修复。结论:冷冻联合激光照射对兔耳静脉有较强的损伤作用,具有治疗静脉畸形的可能性。

Effects of Liquid Nitrogen Cryotherapy Combined with Nd:YAG Laser on Auricular Veins of Rabbit

Objective: This study was designed to observe the effects of liquid nitrogen cryotherapy combined with Nd:YAG laser radiation on veins in rabbit ears,so as to assess its feasibility in the treatment of venous malformations. Methods: Sixty-five rabbits were randomly divided into 4 groups:Group A,Group B and Group C were experimental groups(n=20),Group D was a blank control group(n =5).The central postauricolar veins of rabbits were taken as the models. Group A,veins were frozen with liquid nitrogen and immediately irradiated by Nd:YAG laser;Group B,veins were frozen by liquid nitrogen only;Group C,veins were irradiated by Nd:YAG laser only.Group D,veins retained in blank control. After 1,3,7,14 and 21 days of disposal,the veins were examined under gross observation,light microscopy,and transmission electron microscopy(SEM).Results: In Group A,damages to endothelial cells,vascular smooth muscles and the architecture of structures of veins were obvious,thrombosis appeared and finally resulted in venous obstruction.Group B and Group C,vascular endothelial cell injuries and thrombosis could be found,but thrombosis was gradually dissolved and veins were repaired.Conclusion: Liquid nitrogen cryotherapy combined with Nd:YAG laser radiation could result in major injury to the auricular veins of rabbits and may be applied for venous malformation treatment.