CyclinD1和p27kipl在胰腺癌中的表达与意义

目的　探讨CyclinD1和p2 7kipl在胰腺癌组织中的异常表达及其与临床和病理特征的关系。方法　应用免疫组化技术(SABC法 )检测 42例胰腺癌、2 0例正常胰腺及 15例急慢性胰腺炎组织CyclinD1和p2 7kipl的表达。结果　CyclinD1阳性表达率在胰腺癌组织中为 71 43 % ,显著高于正常胰腺 (0 )和胰腺炎组织 (40 % ) (P <0 0 5)。CyclinD1过表达与病人年龄、性别、肿瘤发生部位、组织分化程度、临床分期及淋巴结转移无关 (P >0 0 5)。p2 7kipl蛋白阳性表达在胰腺癌组织为 45 2 4% ,显著低于胰腺炎组织 (86 67% )(P <0 0 5) ,并与胰腺癌组织分化程度、临床分期及淋巴结转移相关 (P <0 0 5) ,与年龄、性别和肿瘤发生部位无关。两种蛋白之间无明显相关 (P >0 0 5)。结论　胰腺癌组织中CyclinD1过表达 ,p2 7kipl表达下降。CyclinD1和p2 7kipl与胰腺癌发生、发展密切相关

The significance and expression of CyclinD1 and p27kipl in pancreatic carcinoma

Objective To investigate the clinical significance of expression of CyclinD 1 and p27 kipl in pancreatic carcinoma. Methods Immunohistochemical technique(SABC) was applied to detect the expression of CyclinD 1 and p27 kipl in 42 specimen s of pancreatic carcinoma, 15 of pancreatitis and 20 of normal pancreatic tissue. ResultsThe Positive rate of CyclinD 1 protein in pancreatic carcinoma was 71 43%, much hig her than that in pancreatitis and normal pancreatic tissue (P<0.05),p27 kipl pro tein positive rate in pancreatic carcinoma was 45 24%, lower than that in pancreatitis (P <0 05), p27 kipl protein positive-expression was correlated signincantly with tumor cell differe tiation, clinical stage and lymph node metastasis (P<0 05). There was no significant correlat ion between the two proteins. Neither of them was correlated with the age, gender, tumor site. Conclusions The expression of CyclinD 1 in pancreatic carcinoma tissue is s ignincantly increased but the expression of p27 kipl is decreased.CyclinD 1 and p27 kipl might pla y an important role in the generating and progress of pancreatic carcinoma.