多孔聚醚砜平板膜培养肝细胞的初步研究

目的初步观察一种多孔聚醚砜平板膜上支持人肝细胞系L02细胞生长能力,探讨聚醚砜膜材料用于生物人工肝的可行性。方法将正常人肝细胞系L02细胞接种在多孔聚醚砜平板膜片上,用倒置相差显微镜、激光共聚焦显微镜以及扫描电镜观察L02细胞在此种平板膜上的贴壁和生长情况。结果倒置相差显微镜下可以观察到L02细胞能够在多孔聚醚砜平板膜较好生长,细胞培养到7 d时,细胞仍然保持良好生长增殖状态。激光共聚焦显微镜证实了该结果,且随着培养时间延长,细胞可呈多细胞聚集状态生长,保持活性。扫描电镜下膜表面微孔和微小孔结构得以观察,并能观察到部分细胞可以向微孔中延伸密集生长。结论多孔聚醚砜平板膜能够有效的支持L02细胞在其表面生长,有望成为生物人工肝支持系统理想的生物支架材料。

Culturing human hepatocyte L02 on porous polyether sulfone flat membrane

Objective To examine the ability of a novel material of porous flat membrane made of polyether sulfone(PES) to support the growth of human hepatocyte L02.Methods Human hepatocyte L02 was added to PES fiber and then investigated for adherence,growth,spread and survival by invert phase contrast microscope,confocal laser scanning microscope and scanning electron microscope.Results Human hepatocyte L02 was seen growing well in porous PES flat membrane in 7 d.The results by confocal laser scanning microscope coincided with that by invert phase contrast microscope.With time prolonged,hepatocyte spheroids formed.Pores and micropores were observed by scanning electron microscope.Conclusion The PES flat membrane we used supported the growth of human hepatocyte L02 and may serve as a novel scaffolding biomaterial in bioartificial liver.