环氧化酶2和癌胚抗原在结肠癌分子病理研究中的作用

目的 研究距结肠癌不同距离的癌旁组织中环氧化酶2(COX-2)和癌胚抗原(CEA)的表达情况.方法 收集42例行结肠癌根治术患者的组织标本,分别采集新鲜结肠癌组织,远、近端癌旁1、2和3 cm的肠壁组织和距肿瘤8 cm正常肠壁组织,应用RT-PCR技术检测CEA、COX-2在上述组织中的表达情况.结果42例结肠癌肿瘤旁远、近端3 cm处肠壁组织病理检测均为阴性.CEA mRNA、COX-2 mRNA的相对含量由结肠癌组织到远、近端癌旁1、2和3 cm肠壁组织及到距肿瘤8 cm正常结肠组织呈逐渐减少趋势.远、近端癌旁3cm CEA mRNA(分别为18.2±7.9和16.2±6.5)和COX-2 mRNA(分别为21.2±10.3和18.3±7.6)明显低于肿瘤组织(分别为135.2±23.3和134.9±31.1),差异有统计学意义(P＜0.01)；但与正常组织(分别为16.6±7.0和17.1±6.3)比较,差异无统计学意义(P＞0.05).在CEA阳性表达的40例结肠癌组织中,有30例存在COX-2的表达,Pearson相关分析显示,两者呈正相关(r=0.725,P＜0.01).结论 COX-2和CEA可作为结肠癌分子边缘研究的分子标记物.

Rnle of carcinoembrvonic antigen and cvclooxygenase-2 in the study of molecule incisal edge for colorectal cancer

Objective To investigate the expression of cyclooxygenase-2 (COX-2) and CEA in the tissues adjacent to the tumor within different distances.Methods A total of 42 colorectal cancer tissues were collected.The adjacent tissues within 3 cm to the tumor were procured every 1 cm.Normal tissue was also collected.RNA was extracted and the expression of CEA and COX-2 was detected by RT-PCR.Results The CEA mRNA levels of the tumor,the tissues of every 1 cm adjacent to the tumor,and the normal tissue were 135.2±23.3,78.2±17.3,75.9±16.5,56.2±10.7,52.3±12.8,18.2±7.9,16.2±6.5,and 16.6±7.0.The levels of COX-2 mRNA in above positions were 134.9±31.1,79.2±20.2,77.0±20.5,62.7±21.9,58.0±18.1,21.2±10.3,18.3±7.6,and 17.1±6.3.These data showed a decreasing trend of CEA and COX-2 as the distance increased from the tumor.The CEA mRNA levels showed positive correlation with the levels of COX-2 mRNA (r=0.725,P＜0.01).Conclusion CEA and COX-2may be considered to be used as biomarkers for the study of molecular resection margin of colorectal cancer.