人类皮层肌动蛋白基因siRNA干预结肠癌细胞侵袭能力的研究

目的 探讨通过慢病毒载体介导的人类皮层肌动蛋白基因( EMSl) siRNA沉默皮层肌动蛋白( Cortactin)的表达对结肠癌细胞侵袭能力的影响.方法 构建针对Cortactin的编码基因EMS1的siRNA慢病毒载体转染结肠癌细胞HCT8,分为3组:EMS1-siRNA转染组、空载体组,以及未转染组.Wester...

Interference of invasion and metastasis of colorectal cancer cells by EMS1-siRNA encoded by a recombinant lentiviral vector

Objective To explore the effect of Cortactin on invasion and metastasis of human colorectal cancer cells through silencing the expression of Cortactin by transfecting colorectal cancer cells with EMS1-siRNA encoded by a recombinant lentiviral vector.Methods EMS1-siRNA lentiviral vector and negative control lentiviral vector were both transfected into HCT8 cells after virus packing,then the expression of Cortaction of the cells was examined by Western blot to determine the inhibited level of Cortactin.The cells were defined as three groups:EMS1-siRNA transfecting cells,negative control transfecting cells and untransfecting cells.Cell invasion was evaluated with Transwell body.In addition,cells metastasis was observed in animal models.Thirty nude mice were evenly divided into three groups and all the mice were injected through tail vein with 1×107 cells/0.2 ml per animal for each group with the three kinds of cells.The mice were sacrificed 6 weeks after injection and examined for lung metastases development.Results The expression of Cortactin and the ability of invasion of the EMS1-siRNA transfecting cells were all decreased.Lung metastasis rates of EMS1-siRNA transfeting group,negative control group and untransfecting group were 20％,80％ and 80％,respectively.Conclusion siRNA silencing of Cortactin expression can decrease the colorectal cancer cells invasion,and can also decrease the cells' implantation metastasis in animals.