急性炎症状态对家兔急性心肌缺血的影响

目的:探讨急性炎症状态下发生心肌缺血时对心脏的影响.方法:24只成年家兔(1.8～2.5kg)随机分为空白对照组(A),单纯心肌缺血组(B),内毒素组(C),内毒素加心肌缺血组(D),建立AMI模型,术后6h观察血液学指标及缺血心肌的HE染色、COX-2多克隆抗体免疫组织化学染色.结果:血液学指标示术后6hMDA,NO,髓过氧化物酶(MPO)测定值D组与A,B,C组有明显差异(P<0.05);后3组心房钠尿肽释放增加,D组与B,C组有明显差异(P<0.05),D组左室舒张末压、左室收缩峰压测定值较B,C组降低明显,缺血心肌中环氧化酶-2高度表达.结论:机体处于炎症状态时发生心肌缺血,炎症因子损害心肌,恶化心脏功能.

Effects of experimental acute myocardial infarction (AMI) on rabbits with systemic inflammation

AIM: To investigate the effects of experimental acute myocardial infarction (AMI) on rabbits with systemic inflammation. METHODS: Adult rabbits (weight: 1.8-2.5 kg; n =24) were randomly divided into 4 groups: group A: normal controls; group B: myocardial ischemia: The left circumflex arteries of rabbits were ligated to establish the animal models of AMI; group C: Lipopolysaccharide (LPS) and group D: myocardial ischemia + LPS. Six hours after AMI, the serum MDA, NO, MPO and ANP were measured and myocardial HE and COX 2 expression were determined by immunohistochemical staining. RESULTS: Plasma MDA, NO and MPO increased significantly 6 h after AMI in groups B, C and D, and those in group D increased more significantly compared with those in groups B and C( P < 0.05) . ANP and LVEDP and LVPEP significantly increased in group D compared with those of group B( P <0.05). In group D, the myocardial COX 2 expression was significantly higher than that in group B and C. CONCLUSION: Inflammatory factors damage the myocardium and aggravate the heart function.