心灵丸中三七皂苷R1、人参皂苷Rg和Rb1的含量测定

目的建立同时测定心灵丸中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1含量的高效液相色谱（HPLC）法。方法色谱柱为Agilent Hypersil ODS柱（250mm×4．0mm，5μm），流动相A为乙腈，B为水，梯度洗脱（0min 19％A→12min 19％A→60min 36％A），流速为1．0mL／min，检测波长为203nm。结果三七皂苷R1进样量在0．1378～2．7560μg范围内与峰面积线性关系良好，r=0．9997，平均回收率为98．95％，RSD为0．67％；人参皂苷Rg1进样量在0．5672-11．3440μg范围内与峰面积线性关系良好，r=0．9999，平均回收率为99．10％，RSD为0．29％；人参皂苷Rb1进样量在0．4186～8．3720μg范围内与峰面积线性关系良好，r=0．9999，平均回收率为99．02％，RSD为0．42％。结论HPLC法简便准确、专属性强，可用于心灵丸的质量控制。

Determination of Notoginsenoside R1 and Ginsenoside Rg1, Rb1 in Xinling Pills by HPLC

Objective To establish a HPLC method for the content determination of notoginsenoside R1 and ginsenoside Rg1, Rb1 in Xinling Pills. Methods The analysis was performed on the Agilent Hypersil ODS column（250 mm × 4.0 mm,5 μm）, using the mixture of acetonitrile （A） and water（B） as the mobile phase. Elution was performed using the gradient mode（0 min 19% A→12 min 19% A→60 rain 36% A）, and the flow rate was 1.0 mL/min. The detection wavelength was 203 nm. Results There was good linearity in the range of 0. 137 8- 2. 756 0 μg for notoginsenoside R1 （ r = 0. 999 7）, 0. 567 2 - 11. 344 0 μg for ginsenoside Rg1 （ r = 0. 999 9） and 0. 418 6 - 8. 372 0 μg for ginsenoside Rb1（r=0. 999 9）.The average recovery rates and RSD were 98.95% and 0. 67% for notoginsenoside R1, 99.10% and 0. 29% for ginsenoside Rg1 and 99.02% and 0. 42% for ginsenoside Rb1. Conclusion This method is simple and reliable for the quality control of Xinling Pills.