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Extended antimicrobial resistance screening of the dominant faecal Escherichia coli and of rare resistant clones |
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| Authors: | Kronvall Göran Larsson Mattias Borén Charlotte Kahlmeter Gunnar Bartoloni Alessandro Rossolini Gian Maria Grape Malin Kristiansson Charlotte Karlsson Inga |
| Affiliation: | aClinical Microbiology – MTC, Karolinska Institute, Karolinska Hospital L2:02, SE-17176 Stockholm, Sweden bDepartment of Public Health Sciences, Division of International Health (IHCAR), Karolinska Institute, SE-17176 Stockholm, Sweden cDepartment of Clinical Microbiology, Växjö Central Hospital, SE-35185 Växjö, Sweden dDipartimento di Area Critica Medico Chirurgica, Clinica di Malattie Infettive, Università di Firenze, Firenze, Italy eDipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, Siena, Italy |
| Abstract: | Fifty faecal samples from healthy adults were grown on MacConkey agar and three pink colonies were subcultured, identified to species level and their antimicrobial susceptibility determined. Forty-seven samples yielded 141 isolates of Escherichia coli that were susceptible to most antimicrobials. Resistance was noted for ampicillin (30.5%), chloramphenicol (12.1%), tetracycline (23.4%), trimethoprim (24.8%) and co-trimoxazole (22.7%). A direct faecal plating method was used for extended resistance screening with E. coli as the indicator organism. Zone breakpoints were determined using normalised resistance interpretation and gave similar susceptibility results. Eighty-eight isolates of E. coli from within the zones of inhibition revealed four times more antimicrobial resistance. Extended antimicrobial resistance screening both provides the susceptibility profile of the dominant E. coli isolate and detects greater resistance in rare isolates. |
| Keywords: | Antimicrobial resistance surveillance Escherichia coli Normalised resistance interpretation Interpretive zone breakpoints Disk diffusion test |
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