胃周淋巴结淋巴因子激活的杀伤细胞抗肿瘤研究

淋巴细胞经白细胞介素-2(Interleukin-2,IL-2)体外诱导培养后,可产生明显的杀伤肿瘤细胞活性,此杀伤细胞称为LAK细胞(lymphokine activated killer cells)。本实验取19例胃癌患者胃引流区淋巴结淋巴细胞(gastric drainage lymphnode lymphocytes,GDLNL),在体外经重组白细胞介素-2诱导培养若干天后,对高分化胃腺癌细胞MNK_(28)进行体外杀伤实验。结果提示,无癌转移的淋巴结淋巴细胞在诱导培养3天～2周内,LAK活性较高;而有癌转移的淋巴结淋巴细胞在2周内亦有明显的LAK活性,但较低,以后逐渐升高,3周最高,且至5周时,仍有杀伤肿瘤活性。

Study of Antitumor Activity of Lymphokine-activated Lymphocytes from Gastric Drainage Lymph Nodes

After induce cultured with rIL-2, lymphocytes can kill tumor cells obviously.This killer cells are called lymphokine-activated killer cells (LAK). In this test, ga-stric drainage lymph node lymphocytes from 19 gastric cancer patients were culturedwith rIL-2 in vitro for several days, then they used to kill high differentiated gastricadenocarcinoma ce1l-MNK_(28). The result suggested that the LAK activity of lymphocytesfrom no metastasis lymph nodes were high during 3 days to 2 weeks after cultured.The lymphocytes from metastatic lymph nodes had obvious LAK activity, but it islow at begining, then it became higher and higher. On the 3 rd week it reached thepeak and the activity might last to 5 th week.