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| 甲酸钠抗氧自由基损伤的研究 | |
| 引用本文： | 何文珊,严玉霞,蒋建伟,郭宝江. 甲酸钠抗氧自由基损伤的研究[J]. 广州医学院学报, 2001, 29(1): 8-10 |
| 作者姓名： | 何文珊 严玉霞 蒋建伟 郭宝江 |
| 作者单位： | 1. 暨南大学医学院生化教研室,华南师范大学生物技术研究所, 2. 暨南大学医学院生化教研室, 3. 华南师范大学生物技术研究所, |
| 摘 要： | 目的和方法 :采用溶血试验 ,小鼠组织谷胱甘肽 (GSH)、脂质过氧化产物 (MDA)测定 ,采用质粒构象改变等方法检测甲酸钠的抗氧自由基损伤活性。结果 :甲酸钠明显抑制H2 O2 致人红细胞 (RBC)溶血作用 (P <0 .0 5或P <0 .0 1) ;明显提高小鼠肝GSH水平 (P <0 .0 5 ) ;抑制小鼠肝、肾脂质过氧化的产生 (P <0 .0 5或P <0 .0 1) ;抑制受辐射质粒PUC18超螺旋构象百分率的下降 (P <0 .0 5或P <0 .0 1)。结论 :甲酸钠具有明显抗氧自由基损伤的活性 ,是一个良好的自由基清除剂。 |
| 关 键 词： | 甲酸钠 溶血试验 谷胱甘肽 脂质过氧化 PUC18 自由基清除剂 |
| 文章编号： | 1008-1836(2001)01-0008-03 |
A Study of Sodium Formate in Preventing Oxygen Free Radical Damage |
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| HE Wen shan ,,JIANG Jian wei ,GUO Bao jiang ,YAN Yu xia. A Study of Sodium Formate in Preventing Oxygen Free Radical Damage[J]. Academic Journal of Guangzhou Medical College, 2001, 29(1): 8-10 | |
| Authors: | HE Wen shan JIANG Jian wei GUO Bao jiang YAN Yu xia |
| Affiliation: | HE Wen shan 1,2,JIANG Jian wei 1,GUO Bao jiang 2,YAN Yu xia 1 |
| Abstract: | To investigate the activity of sodium formate (SF) as a free radical scavenger. Methods:The anti-oxygen free radical activity of sodium formate was determined by hemolysis test and measurement of GSH、MDA levels in mice tissue homogenates and the conformation changes of irradiated PUC18. Results:SF significantly reduced the hemolysis level of human RBC induced by H2O2(P<0.05 or P<0.01),increased the GSH levels in mice liver homogenate and decreased the MDA levels in mice liver and kidney homogenates (P<0.05 or P<0.01). SF could prevent the decline of the percentage of the supercoil conformation of plasmid PUC18 irradiated with 120Gy of 60Coγ-rays.(P<0.05 or P<0.01). Conclusion: SF is an oxygen free radical scavenger, it can prevent the oxygen free radical damage. |
| Keywords: | sodium formate hemolysis GSH lipid peroxides PUC 18 |
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