| 兔骨髓基质细胞与自体牙骨质片的体外附着生长 |
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| 引用本文： | 陈明,刘宏伟. 兔骨髓基质细胞与自体牙骨质片的体外附着生长[J]. 医学争鸣, 2004, 25(5): 418-420 |
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| 作者姓名： | 陈明 刘宏伟 |
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| 作者单位： | 第一军医大学南方医院口腔科,广东,广州,510515;第一军医大学南方医院口腔科,广东,广州,510515 |
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| 基金项目： | 军队指令性项目，广东省科技厅科技计划 |
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| 摘 要： | 目的:探索兔骨髓基质细胞与自体牙骨质片的体外附着生长规律. 方法:体外分离培养新西兰大白兔骨髓基质细胞,接种于牙片联合培养4 wk,通过倒置相差显微镜、扫描电镜观察细胞与材料附着情况. 结果:骨髓基质细胞附着牙骨质片生长良好,贴附紧密,并能形成新生的类牙骨质样矿化组织. 结论:骨髓基质细胞能在牙骨质片上附着生长并形成新生矿化组织,是理想的牙周组织工程种子细胞.
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| 关 键 词： | 骨髓基质细胞 牙骨质 组织工程 |
| 文章编号： | 1000-2790(2004)05-0418-03 |
| 修稿时间： | 2003-11-28 |
Adhesion rabbit bone marrow stromal cells to cementum slices in vitro |
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| CHEN Ming,LIU Hong Wei. Adhesion rabbit bone marrow stromal cells to cementum slices in vitro[J]. Negative, 2004, 25(5): 418-420 |
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| Authors: | CHEN Ming LIU Hong Wei |
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| Affiliation: | CHEN Ming,LIU Hong Wei Department of Stomatology,Nanfang Hospital,First Military Medical University,Guangzhou 510515,China |
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| Abstract: | AIM: To investigate growth of rabbit bone marrow stromal cells (BMSC) adhesion to the cementum slices in vitro . METHODS: BMSC of rabbits were isolated and cultured in vitro and then co cultured with the cementum slices. Cell adhesion at different times was analyzed by reverse phase difference microscope and scanning electro microscope. RESULTS: BMSC were well adhered to the cementum slices and the cells on the cementum slices exhibited a cementum like and mineralized appearance. CONCLUSION: BMSC can well adhere to and proliferate on the cementum slices and they can form a new type of mineralized tissue. |
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| Keywords: | bone marrow stromal cells dental cementum tissue engineering |
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