| Agacutase体外水解牛纤维蛋白原机制的研究(英文) |
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| 引用本文： | 刘玉超,周东,李谦,颜丽云,唐松山. Agacutase体外水解牛纤维蛋白原机制的研究(英文)[J]. 药物生物技术, 2012, 0(2): 110-112 |
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| 作者姓名： | 刘玉超 周东 李谦 颜丽云 唐松山 |
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| 作者单位： | 广东药学院基础学院生物化学和分子生物学系;广东省人民医院神经外科;中国药科大学生命科学与技术学院 |
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| 基金项目： | supported by GDPU grant 43755005 and 52104109~~ |
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| 摘 要： | Agacutase是自尖吻蝮蛇蛇毒中分离出的新的具有止血功能的蛇毒类凝血酶,它能够将纤维蛋白原转化为纤维蛋白单体。通过SDS-PAGE和ELISA方法,我们研究了Agacutase体外水解牛纤维蛋白原的分子机制。实验结果显示,Agacutase仅仅水解牛纤维蛋白原的α亚基并释放血纤肽A,而对牛纤维蛋白原的β和/或γ亚基没有影响。研究表明Agacutase属于血纤肽A类(FP-A类型)的类凝血酶。
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| 关 键 词： | 尖吻蝮蛇蛇毒 类凝血酶 Agacutase 纤维蛋白原 酶水解 体外。 |
Hydrolysis Mechanism of Agacutase on Bovine Fibrinogen in Vitro |
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| LIU Yu-chao,ZHOU Dong,LI Qian,YAN Li-yun,TANG Song-shan. Hydrolysis Mechanism of Agacutase on Bovine Fibrinogen in Vitro[J]. Pharmaceutical Biotechnology, 2012, 0(2): 110-112 |
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| Authors: | LIU Yu-chao ZHOU Dong LI Qian YAN Li-yun TANG Song-shan |
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| Affiliation: | 1(1Department of Biochemistry & Molecular Biology,School of Basic Courses,Guangdong Pharmaceutical University,Guangzhou 510006,China;2 Department of Neurosurgery,Guangdong General Hospital,Guangzhou 510080,China;3School of Life Science and Technology,China Pharmaceutical University,Nanjing 210009) |
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| Abstract: | Agacutase from Agkistrodon acutus snake venom is a novel snake venom thrombin-like enzyme(TLE) with haemostatic function.It converts fibrinogen to fibrin.In this study SDS-PAGE and ELISA methods were used to investigate in vitro hydrolysis mechanism of Agacutase on bovine fibri-nogen.Experimental result,showed that Agacutase only hydrolyzed α-subunit of bovine fibrinogen to release fibrinopeptide A(FPA) and did not influence β-and/or γ-subunits of the bovine fibrinogen.The results showed that Agacutase belongs to thrombin-like enzyme of fibrinopeptide A class(FP-A class). |
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| Keywords: | Agkistrodon acutus snake venom Thrombin-like enzymes Agacutase Fibrinogen Enzymatic hydrolysis In vitro. |
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