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Hes1在大鼠脊髓发育不同阶段的表达
引用本文:马杰,江金群,胡建国,张玉心,石莹. Hes1在大鼠脊髓发育不同阶段的表达[J]. 蚌埠医学院学报, 2015, 40(3): 314-317. DOI: 10.13898/j.cnki.issn.1000-2200.2015.03.007
作者姓名:马杰  江金群  胡建国  张玉心  石莹
作者单位:1. 蚌埠医学院 临床检验诊断学实验中心, 安徽 蚌埠 233030;2. 粤北人民医院 检验科, 广东 韶关 512026;3. 蚌埠医学院 组织移植省重点实验室, 安徽 蚌埠 233030;4. 蚌埠医学院 医学检验系, 安徽 蚌埠 233030
基金项目:国家自然科学基金资助项目(81071268);蚌埠医学院科研课题(BYKY1204)
摘    要:目的:检测Hes1在大鼠脊髓发育过程中不同阶段的表达。方法:选取胚胎15.5 d、18.5 d和出生当天,出生后3 d、7 d、2周、4周及成年的SD大鼠脊髓,采用Western blot和荧光定量PCR检测Hes1在大鼠脊髓发育过程中的表达。结果:从胚胎15.5 d到成年期,大鼠脊髓中都有Hes1的表达,具有一定的变化规律,总体上来看,胚胎期表达量比较高,出生后的表达降低。结论:Hes1在大鼠脊髓发育过程中的不同阶段表达量有所不同,胚胎期表达量高,出生后表达量明显下降。

关 键 词:hes1基因   脊髓   大鼠
收稿时间:2013-12-17

Expression of the Hes1 in the development process of rat spinal cord
MA Jie , JIANG Jin-qun , HU Jian-guo , ZHANG Yu-xin , SHI Ying. Expression of the Hes1 in the development process of rat spinal cord[J]. Journal of Bengbu Medical College, 2015, 40(3): 314-317. DOI: 10.13898/j.cnki.issn.1000-2200.2015.03.007
Authors:MA Jie    JIANG Jin-qun    HU Jian-guo    ZHANG Yu-xin    SHI Ying
Affiliation:1. Clinical Laboratory Diagnostics Experiment Center, Bengbu Medical College, Bengbu Anhui 233030;2. Department of Clinical Laboratory, Northern part of Guangdong People's Hospital, Shaoguan Guangdong 512026, China;3. Tissue transplants Provincial Key Laboratory, Bengbu Medical College, Bengbu Anhui 233030;4. Department of Clinical Laboratory Diagnostics, Bengbu Medical College, Bengbu Anhui 233030
Abstract:Objective:To detect the expression of Hes1 in the development process of rat spinal cord. Methods:The rat spinal cord at embryonic day 15. 5 and 18. 5,the day of birth,3 days after birth,7 days,2 weeks and 4 weeks,and adult rat spinal cord were harvested,the Hes1 expressions of which were detected by the fluorescence quantitative PCR and Western blot. Results:The expressions of Hes1 in the rat spinal cord of 15. 5 d to adulthood were found,which showed certain regular changes. The expressions of Hes1 in embryonic stages of rat was high,which was decreased after birth. Conclusions:The expression of Hes1 at different stages of the rat spinal cord development is different,which was high in the embryonic period,and was depressed after birth.
Keywords:hes1  genes spinal cord  rat
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