免疫滴金技术检测肾综合征出血热特异性抗体及中西医结合治疗的研究

目的：探讨一种更为简单，快速，特异，灵敏的肾综合征出血热（HFRS）抗体的检测方法及中西医结合治疗该病的有效手段，方法：采用免疫滴金法（CGIDA）检测HFRS患者186例，同时测定特异性免疫球蛋白M，G抗体（抗HFRS－IgM，抗HFRS－IgG)，其中101例HFRS患者分组进行治疗（治疗组用苦黄注射液，参麦注射液联合黄芪汤治疗，对照组用利巴韦林联合甘利欣注射液治疗）。结果：检测186例HFRS患者中抗HFRS－IgM阳性132例（70．9％），抗HFRS－IgG阳性163例（87．1％），越期情况：越休克期治疗组优于对照组（P＜0．05），越少尿期及从发热期直接进入多尿期，两组差异无显著性。结论：CGIDA检测抗HFRS－IgM，抗HFRS-IgG具有快速，简便之优点，治疗方面两组比较差异无显著性。

Study on Using Colloidal Gold Immuno Dot Assay to Detect Special Antibody of Hemorrhagic Fever Renal Syndrome

To seek for a simple, sensitive and rapid assay to detect specific antibody of patients suffering from hemorrhagic fever renal syndrome (HFRS). Methods: Serum anti HFRS IgM (sIgM) and IgG (sIgG) antibody were detected in 186 patients with HFRS using colloidal gold immuno dot assay (CGIDA), and compared with those detected with enzyme linked immunosorbant assay (ELISA) and indirect fluorescent antibody test (IFAT). Kuhuang Shenmai injection (KHSM) combined Astragalus decoction (AD) treatment was applied to 101 HFRS patients in a treated group (n=50), and the effect of therapy was compared with that in a control group (n=51) treated with ribarvirin and ganlixin injection. Results: CGIDA showed positive sIgM antibody in 132 cases (70.9%) and positive sIgG antibody in 163 (87.1%) of the 186 HFRS patients. As compared the two groups in crossing stage, the rate of crossing shock stage in the treated group was higher than that in the control (P<0 05). As for rate of crossing oliguria stage and from febrile stage directly developed into diuretic stage, the difference between the two groups was insignificant (P<0 05). Conclusion: CGIDA shows the merits of quick, simple and convenient in detecting sIgM and sIgG antibody in HFRS patients. The effect of KHSM combined AD treatment was not significantly different from that of ribarvirin and ganlixin injection.