凋亡抑制蛋白XIAP在喉鳞状细胞癌中的表达及其临床病理学意义

目的:探讨调亡抑制蛋白XIAP在喉癌中的表达情况,了解其与喉癌临床生物学行为的关系。方法:选择喉鳞状细胞癌患者50例,术前均未进行化学和放射治疗。采用免疫组织化学方法染色(SP法),检测XI-AP蛋白在喉癌和喉正常黏膜组织中的表达及其与临床病理参数之间的关系。结果:XIAP蛋白主要表达在肿瘤细胞细胞质和细胞核,为粗细不一的棕黄至棕黑色颗粒。XIAP在喉鳞状细胞癌中的表达与正常喉黏膜比较,前者表达显着增高,差异有统计学意义(P<0.01)。XIAP蛋白阳性表达与患者年龄、性别、吸烟史、肿瘤生长部位及有无淋巴结转移无相关性,而与临床分期、T分期及病理分级有关(均P<0.05)。结论:XIAP蛋白在喉正常组织中低表达,而在喉癌中高表达。XIAP蛋白的表达程度与肿瘤临床病理特征有关。XIAP在喉癌发生、发展过程中可能具有重要作用。

Expression of inhibitor of apoptosis protein XIAP in laryngeal carcinoma and its clinocopathologicai significance

Objective:To investigate the expression of X-chromosome-linked inhibitors of apoptosis(XIAP)XIAP in laryngeal squamous carcinomas and the relationship between the expression of XIAP and clinical biological behaviors.Method:Paraffin-embedded tissue specimens used for this study were obtained from 50 patients with laryngeal squamous carcinomas.The patients had received neither chemotherapy nor radiation therapy before tumor resection.Using immunohistochemical staining for the paraffin sections(SP methods),we examine the expression of XIAP protein in laryngeal squamous carcinomas and normal laryngeal tissues,investigate the connection of the XIAP expression with the clinicopathological parameters.Result:The expression of XIAP protein was observed mainly in the cytoplasm and nucleus.The staining color was dark brown.The expression of XIAP is remarkably higher in laryngeal squamous carcinomas than that in normal laryngeal tissue specimens.The statistical analysis revealed that in laryngeal squamous carcinomas XIAP expression had no relationship with the elements such as age,sex,smoking history,tumor site and lymph node metastases.However,there is significant correlation between XIAP expression and tumor clinical stage,T stage and pathological stage(P<0.05).Conclusion:XIAP is expressed higher in laryngeal squamous carcinomas than in normal laryngeal tissues.The level of XIAP expression is associated with tumor clinicopathological characteristics in laryngeal squamous carcinomas.While tumor growth and malignancy increased,the expression of XIAP was up-regulated in laryngeal squamous carcinomas.It may play a role of anti-apoptosis in the process of carcinogenesis and development in laryngeal squamous carcinomas.