| 阳离子脂质体包裹的IL-12基因对小鼠黑色素瘤的治疗作用 |
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| 引用本文： | 陈玮,邵传森,沈建根,潘毅,郑树.阳离子脂质体包裹的IL-12基因对小鼠黑色素瘤的治疗作用[J].浙江大学学报(医学版),2005,34(1):73-76. |
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| 作者姓名： | 陈玮 邵传森 沈建根 潘毅 郑树 |
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| 作者单位： | 1. 浙江大学医学院,免疫教研室,浙江,杭州,310031
2. 浙江大学医学院,附属第二医院肿瘤研究所,浙江,杭州,310009 |
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| 摘 要： | 目的:观察体内注射阳离子脂质体包裹的小鼠白细胞介素12(mIL-12)基因对小鼠黑色素细胞瘤的治疗作用.方法:C57BL/6小鼠在接种B16黑色素细胞后3、5、7、9 d分别给予lipofectin包裹的pCmIL-12质粒治疗,观察小鼠的肿瘤生长速度、生存期以及NK细胞活性的变化.结果:阳离子脂质体明显增强pCmIL-12质粒在体内的抗肿瘤生长活性,并延长荷瘤小鼠的存活期.此外,pCmIL-12/阳离子脂质体还能显著增强荷瘤小鼠的NK细胞活性.结论:阳离子脂质体介导的IL-12基因对小鼠黑色素瘤有明显的治疗效果.
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| 关 键 词： | 白细胞介素12/治疗应用 阳离子脂质体/治疗应用 黑色素瘤/药物疗法 杀伤细胞 |
| 文章编号： | 1008-9292(2005)01-0073-04 |
| 修稿时间： | 2004年6月30日 |
Therapeutic effect of cationic liposome-mediated interleukin-12 gene delivery on murine melanoma in vivo |
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| CHEN Wei,SHAO Chuan sen,SHEN Jian gen,et al.Therapeutic effect of cationic liposome-mediated interleukin-12 gene delivery on murine melanoma in vivo[J].Journal of Zhejiang University(Medical Sciences),2005,34(1):73-76. |
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| Authors: | CHEN Wei SHAO Chuan sen SHEN Jian gen |
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| Institution: | Institute of Immunology, College of Medicine, Zhejiang University, Hangzhou 310031, China. |
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| Abstract: | Objective: To investigate the therapeutic effect of cationic liposome mediated interleukin 12 gene delivery on established murine melanoma in vivo. Methods: The lipofectin encapsulated pCmIL 12 plasmid was given to C57BL/6 mice on the day 3,5,7,9 after inoculation of B16 melanoma cells.The tumor size,the survival time of mice and the NK cell activity were observed. Results: The pCmIL 12 plasmid coupled with cationic liposome inhi bited the tumor growth and improved the survival of mice bearing established melanoma.The activity of NK cells was also enhanced after interleukin 12 gene delivery in vivo . Conclusion: WTBZ Cationic liposome mediated interleukin 12 gene delivery has significantly therapeutic effects on mice melanoma in vivo. |
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| Keywords: | Interleukin-12/ther use Cationic liposome/ther use Melanoma/drug ther Killer cell |
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