用双色荧光原位杂交法检测苯系物接触工人精子染色体数目 …

目的　探讨苯系物对男工精子性染色体数目畸变的影响。方法　用X、Yα 卫星染色体特异DNA探针与苯系物接触男工间期精子核进行双色荧光原位杂交 ,同时测定X和Y精子染色体非整倍体率。结果　车间空气中苯浓度几何均数 83 95mg m3 高于国家最高容许浓度 1倍。对 13位苯系物作业工人共计数 74 5 17条精子 ,X、Y和XY精子双体率分别为 ( 0 15± 0 0 5 ) %、( 0 13± 0 0 5 ) %和( 0 2 0± 0 11) % ;13位正常对照工人共计数 89662条精子中X、Y及XY精子双体率分别为 ( 0 10±0 0 4 ) %、( 0 10± 0 0 4 ) %和 ( 0 13± 0 0 6) %。接触组精子X双体率及总双体精子率高于对照组。结论　高浓度苯接触可以诱导接触工人精子性染色体非整倍体率增加。

Detection of numerical chromosome aberrations in sperm of workers exposed to benzene series by two-color fluorescence in situ hybridization

OBJECTIVE: To study the numerical aberrations of sex chromosomes in sperm of workers exposed to benzene series. METHODS: The interphase sperm from workers of exposed and control groups were hybridized using two color fluorescence in situ hybridization with X and Y(alpha)-satellite chromosome specific DNA probes, and the aneuploidy frequencies of X and Y chromosome in X and Y sperms were detected simultaneously. RESULTS: Geometric mean concentration of benzene in workplace air was 83.95 mg/m(3), one fold higher than national maximum allowable concentration. A total of 74,517 sperm nuclei in 13 benzene-exposed workers and 89,662 sperm nuclei in 13 control workers were counted. Frequencies of disomic sperm for X, Y and XY were (0.15 +/- 0.05)%, (0.13 +/- 0.05)% and (0.20 +/- 0.11)% in workers exposed to benzene series, and (0.10 +/- 0.04)%, (0.10 +/- 0.04)% and (0.13 +/- 0.06)% in control workers. There was statistical difference in disomic frequency of X sperm and overall disomic frequency between the benzene series-exposed workers (0.15% and 0.48%, respectively) and the control workers (0.10% and 0.33%, respectively). CONCLUSION: Exposed to benzene at higher concentration may induce increase in aneuploidy frequency of sperm sex chromosome in exposed workers.