白蛋白钴结合试验测定缺血修饰白蛋白方法学评价

目的白蛋白钴结合（ACB）试验测定缺血修饰白蛋白（IMA），并确定参考正常范围。方法应用日立7600—120E型全自动生化分析仪，采用ACB法测定IMA。结果ACB法批内变异系数（CV）为1．43％，批间CV为1．70％。不同浓度的IMA结果线性相关，回归方程为Y=-13．752X＋83．835，r=0．9586。脂血（TG≤20．5mmol／L）、黄疸（TBIL≤259．6），对测定无影响，溶血对IMA测定影响较大。健康人群的参考范围为≥62．84U／ml，心肌缺血患者为54．49U／ml。结论IMA是诊断心肌缺血的有效工具，应用全自动生化分析仪检测IMA快速、准确、简便。

Detection of Ischemia-modified albumin by Cobalt binding test

Objective In the present study,the ischemia-modified albumin (IMA) in serum samples was detected by the ACB method,and its reference range was also determined.Methods IMA was determined by the albumin cobalt binding (ACB) test using the Hitachi 7600-120E automatic biochemical analyzer.Results Coefficient of variation (CV) for within-run assays was 1.43%;CV for between-run assays was 1.70%.Measurement of ACB using different concentrations of IMA was linear,regression equation was Y=-13.752X+83.835,r=0.9586,P...