Semi-automatic DNA profiling in a Hungarian Romany population using the STR loci HumVWFA31, HumTH01, HumTPOX, and HumCSF1PO |
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| Authors: | S Füredi M Angyal Z Kozma J Sétáló J Woller Z Pádár |
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| Institution: | (1) Department of Biology, Institute for Forensic Sciences, P.O. Box 314/4, H-1903 Budapest, Hungary, HU;(2) Department of Forensic Medicine, Medical University School of Pécs, P.O. Box 99, H-7643 Pécs, Hungary, HU |
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| Abstract: | A population study of Hungarian Romanies was carried out for the STR loci HumVWFA31, HumTH01, HumTPOX, and HumCSF1PO. After
multiplex PCR amplification semi-automatic DNA profiling was performed using an ALF DNA sequencer. At the loci investigated
there was little and no evidence for departures from Hardy-Weinberg expectations and linkage equilibrium, respectively. The
allele sizing accuracy of the ALF DNA sequencer was increased to a high level (99.97% on average) by applying external and
internal markers. Allele frequency distributions of the STR loci, with one exception, were significantly different between
the Romany and other Hungarian population databases. On the other hand, however, only small differences in frequencies of
individual phenotypes were found.
Received: 30 December 1996 / Received in revised form: 11 March 1997 |
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| Keywords: | PCR Multiplex STR profiling Automated DNA sequencer Romany (Gypsy) population Hungary |
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