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Stimulatory effects of nitric oxide donors on gastric acid secretion in isolated mouse stomach |
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| Authors: | Ko Hasebe Syunji Horie Midori Komasaka Shingo Yano Kazuo Watanabe |
| Affiliation: | a Laboratory of Chemical Pharmacology, Department of Drug Evaluation and Toxicological Sciences, Faculty of Pharmaceutical Sciences, Chiba University, Chiba 263-8522, Japan b Department of Molecular Pharmacology and Pharmacotherapeutics, Faculty of Pharmaceutical Sciences, Chiba University, Chiba 263-8522, Japan |
| Abstract: | We previously reported on the stimulatory role of endogenous nitric oxide (NO) in gastric acid secretion. In the present study, we investigated the effects of NO donors on acid secretion in isolated mouse stomach. Nitroprusside (100 μM–1 mM) inhibited the gastric acid secretion induced by histamine (500 μM) in a concentration-dependent manner. In addition, nitroprusside abolished the acid secretion induced by bethanechol (100 μM) and by electrical stimulation (10 Hz) of the vagus nerve. On the other hand, nitroprusside, 75 μM, which did not affect the acid secretion induced by histamine, itself elicited an increase in acid secretion. The acid secretion induced by 75 μM nitroprusside was inhibited by 10 μM famotidine, a histamine H2 receptor antagonist. These results suggest that NO donors at high doses act on gastric parietal cells, resulting in inhibition of the stimulated acid secretion, and, at lower doses, facilitate histamine release from histamine-containing cells, leading to the increased acid secretion. |
| Keywords: | Nitric oxide (NO) Gastric acid secretion Histamine Enterochromaffin-like cell Stomach, isolated, mouse |
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