| 高效液相色谱法测定复方茶碱麻黄碱片中可可碱的含量 |
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| 引用本文： | 刘海侠,吴庆淼.高效液相色谱法测定复方茶碱麻黄碱片中可可碱的含量[J].中国中医药信息杂志,2009,16(3):49-50. |
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| 作者姓名： | 刘海侠 吴庆淼 |
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| 作者单位： | 松原市药品检验所,吉林,松原,138000 |
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| 摘 要： | 目的建立复方荼碱麻黄碱片中可可碱的含量测定方法。方法采用高效液相色谱法，以Kromasil C18（4．6mm×250mm，5μm）为色谱柱；流动相为0．05mol／L磷酸二氢钾溶液-甲醇-三乙胺（77：23：0．2）；检测波长为215nm；柱温为45℃；流速为0．8mL／min。结果可可碱在0．3094～0．722μg（r=0．99996）浓度范围内呈良好的线性关系，平均回收率为100．49％，RSD=1．1％。结论本法可准确、灵敏、快速地测定复方茶碱麻黄碱片中可可碱的含量。
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| 关 键 词： | 复方茶碱麻黄碱片 高效液相色谱法 可可碱 含量测定 |
Determination of Theobromine in Compound Theophylline and Ephedrine Tablet by HPLC |
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| LIU Hai-xia,WU Qing-miao.Determination of Theobromine in Compound Theophylline and Ephedrine Tablet by HPLC[J].Chinese Journal of Information on Traditional Chinese Medicine,2009,16(3):49-50. |
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| Authors: | LIU Hai-xia WU Qing-miao |
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| Institution: | (Institute for Food and Drug Control of Songyuan, Songyuan 138000, China) |
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| Abstract: | Objective To establish a HPLC method for determination of theobromine in Compound Theophylline and Ephedrine Tablet. Method HPLC method was adopted on Kromasil C18 column （250 mm×4.6 mm, 5 μm）, and temperature was set at 45 ℃. The mobile phase consisted of 0.05 mol/L KH2PO4- Methanol- Triethanolamine （77 ： 23 ： 0.2）, with flow rate of 0.8 mL/min. The UV detection wavelength was set at 215 nm. Results The calibration curves of theobromine was linear in the range of 0.3094- 0.722 ℃g （r=0.999 96）, ant the average recovery was 100.49% （n=9） with RSD=1.1%. Conclusion This method is simple, rapid, sensitive and accurate, and can be used for determination of theobromine in Compound TheophyUine and Ephedrine Tablet. |
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| Keywords: | Compound TheophyUine and Ephedrine Tablet HPLC theobromine content determination |
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