未成熟树突状细胞表面CD1d分子在体外移植免疫中的作用☆

背景：树突状细胞具有双向免疫调节作用，成熟树突状细胞激活免疫应答，而未成熟树突状细胞则倾向诱导免疫耐受。 目的：探讨鼠CD1d分子在未成熟树突状细胞诱导移植免疫耐受中的作用，以及在此过程中细胞因子的参与机制。 方法：利用vIL-10转染的BALB/c小鼠未成熟树突状细胞在体外用粒细胞-巨噬细胞集落刺激因子基因、脂多糖刺激成熟，经抗CD1d(anti-CD1d)干预。观察培养后的细胞表型、细胞因子表达。为探明CD1d分子在异体T细胞存在下的免疫作用，进行不同实验条件的初次、再次混合淋巴细胞培养(1stMLC、2ndMLC)，观察T细胞增殖、细胞因子表达。 结果与结论：Anti-CD1d的干预降低了未成熟树突状细胞增殖异体T细胞的能力，anti-CD1d干预的未成熟树突状细胞致敏异体T细胞后其免疫功能低下；anti-CD1d干预在异体T细胞存在时影响未成熟树突状细胞在功能上的成熟，主要表现在抑制白细胞介素12的分泌，加强白细胞介素10的分泌。

Contribution of mouse CD1d to immune toleration induced by immature dendritic cells in vitro

BACKGROUND: Dendritic cells (DCs) possess two-way immune regulation ability: mature DCs (mDCs) can activate the immune response, and immature DCs (imDCs) tend to induce immune tolerance. OBJECTIVE: To discuss the role of CD1d in immune toleration induced by imDCs after transplantation and the mechanism of cytokine played in this process. METHODS: imDCs of BALB/c mouse transfected by viral interleukin-10 were stimulated with GM-CSF, LPS in vitro, and separated into three groups after anti-CD1d intervention. The expression of cytokine and phenotypes of the cells were observed. 1st MLC and 2nd MLC were performed in different experimental conditions to observe the immunity of CD1d with heterogeneity T lymphocyte existing. Proliferation of T lymphocyte and the expression of cytokine were observed. RESULTS AND CONCLUSION: Proliferation of heterogeneity T lymphocyte induced by imDC was suppressed by anti-CD1d intervention. The immune function of heterogeneity T lymphocyte was deficient after sensitized by imDCs with anti-CD1d intervention. Anti-CD1d intervention would affect the functional maturity of imDCs in case the heterogeneity T lymphocyte existed, in that it could suppress the secretion of Th1 type cytokine interleukin-12, however enhance the secretion of Th2 type cytokine interleukin-10.