Circulating RNA Profiling in Postreperfusion Plasma From Kidney Transplant Recipients |
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| Institution: | 1. Department of Animal Biotechnology, Kyungpook National University, Sangju, Gyeongsangbuk-do, Republic of Korea;2. School of Medicine, Sungkyunkwan University, Gangnam-gu, Seoul, Republic of Korea;3. Gennbio Co Ltd, Gangnam-gu, Seoul, Republic of Korea;4. Department of Surgery, Samsung Medical Center, Seoul, Republic of Korea;5. Transplantation Research Center, Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, Seoul, Republic of Korea;6. Department of Health Sciences & Technology, Samsung Advanced Institute for Health Sciences & Technology, Graduate School, Sungkyunkwan University, Seoul, Republic of Korea;7. Graduate School of International Agricultural Technology, Seoul National University, Gangwon-do, Republic of Korea;1. Yeni Yuzyil University, School of Medicine, Private Gaziosmanpasa Hospital, Department of General Surgery and Organ Transplantation, Istanbul, Turkey;2. Yeni Yuzyil University, School of Medicine, Private Gaziosmanpasa Hospital, Department of Interventional Radiology, Istanbul, Turkey;3. Yeni Yuzyil University, School of Medicine, Private Gaziosmanpasa Hospital, Department of Anesthesiology and Reanimation, Istanbul, Turkey;4. Yeni Yuzyil University, School of Medicine, Private Gaziosmanpasa Hospital, Department of Gastroenterology and Organ Transplantation, Istanbul, Turkey;1. Internal Medicine Department, Loma Linda University Medical Center, Loma Linda, California;2. Cardiology Department, Loma Linda University Medical Center, Loma Linda, California;1. Department of Liver Transplantation and Hepatobiliary Surgery, Ajou University School of Medicine, Suwon, Republic of Korea;2. Department of Pediatric Surgery, Ajou University School of Medicine, Suwon, Republic of Korea;1. Department of Medicine, University of Southern California, Los Angeles, California;2. Saint Barnabas Medical Center, Livingston, New Jersey;3. Natera Inc, San Carlos, California |
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| Abstract: | BackgroundIschemia/reperfusion injury (IRI) is inevitable in kidney transplantation (KT) and may lead to impaired tubular epithelial cell function and reduce graft function and survival. Renal IRI is a complex cellular and molecular event; therefore, investigating the genetic or molecular pathways associated with the early phase of KT would improve our understanding of IRI in KT. MicroRNAs (miRNAs) play a critical role in various pathologic events associated with IRI.MethodsWe compared the expression profile of miRNAs extracted from 2 blood plasma samples, 1 from periphery and the other form gonadal veins immediately after reperfusion, in a total 5 cases of KT.ResultsWe observed that the total RNA yield was higher in postreperfusion plasma and that a subset of miRNAs was upregulated (miR-let-7a-3p, miR-143-3p, and miR-214-3p) or downregulated (let-7d-3p, let-7d-3p, miR-1246, miR-1260b, miR-1290, and miR-130b-3p) in postreperfusion plasma. Gene ontology analyses revealed that these subsets target different biological functions. Twenty-four predicted genes were commonly targeted by the upregulated miRNAs, and gene ontology enrichment and pathway analyses revealed that these were associated with various cellular activities such as signal transduction or with components such as exosomes and membranous organelles.ConclusionWe present 2 subsets of miRNAs that were differentially upregulated or downregulated in postreperfusion plasma. Our findings may enhance our understanding of miRNA-mediated early molecular events related to IRI in KT. |
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