乳胶凝集法快速检测金黄色葡萄球菌肠毒素

Rapid Latex Agglutination Test for Detection of Staphylococcal Enterotoxins

Staphylococcal enterotoxins (SE) play an important role in the pathogenesis of human infection of Staphylococcus aureus. After comparing various sensitzing effects with dif- ferent latex particles and different sensitizing methods,we attempted to develop a reversed passive latex agglutination method (RPLA) for rapid detection of SEA, SEB, SEC and SED. Examined by the method, all the 19 ref- erence enterotoxigenic staphylococcus aureus strains and I non-toxigenic strain showed positive and negative, respectively. We also studied the production of SE by 87 staphylo- coccus aureus strains isolated from hospitals in Beijing, Xian and Jinan. The incidence of entero-toxigenic strains was 85.1%(74/87), which was higher than theincidence of 65.5 (57/87)detected by the double immuno-geld- iffusion (DIGD) and was close to the incidence of 77.0%(67/87)tested by the reversed pas- sive hemagglutination test (RPHA). Of en- terotoxigenic strains studied by the RPLA, ablend of SEA + SEB + SEC + SED was pro- duced by the maximum number of the sterains, and by DIGD and RPHA, SEA(34/ 57, 24/67) was predominant, respectively. The method was shown to be sensitive(mini- mum SE detectabl limit was 6~12ng/ml), highly specific (no agglutination occured with Cholera toxin and cultures of E. coli, Shigel- la, Bacillas subtilis and pseudomonas aerogi- nosa)and rapid (took 5~7h for incubation at room temperature). These showed the RPLA is fully reliable for use.